Invitrogen

GLRA1 Polyclonal Antibody

View all (19) GLRA1 antibodies

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Datasheet

Protocols

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GLRA1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of GLRA1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GLRA1 Polyclonal Antibody (Product # PA5-28362) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

GLRA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Glycine Receptor alpha1 (Product # PA5-28362) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

GLRA1 Antibody in Western Blot (WB) — Western Blot using GLRA1 Polyclonal Antibody (Product # PA5-28362). Sample (30 µg of whole cell lysate). A: HeLa S3. 7.5% SDS PAGE. GLRA1 Polyclonal Antibody (Product # PA5-28362) diluted at 1:1500.

Product Details

PA5-28362

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 35 and 256 of Human Glycine Receptor alpha1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.43 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545838

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GLRA1 is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in GLRA1 are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found for GLRA1. Glycine receptors (GlyR) that include GLRA1 are members of the cys-loop family of ligand-gated ion channels, responsible for mediating the inhibitory effects of glycine. GLRA1 are widely distributed throughout the CNS, particularly within the hippocampus, spinal cord and brain stem. Diseases associated with GLRA1 include Hyperekplexia, Hereditary 1 and Hyperekplexia.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GLRA; Glra2; glycine receptor; Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1; glycine receptor, alpha 1 subunit; gra1

Gene Aliases: GLRA1; Glyr; GLYRA1; HKPX1; STHE

UniProt ID: (Human) P23415, (Rat) P07727

Entrez Gene ID: (Human) 2741, (Rat) 25674

Molecular Function: ligand-gated ion channel