MM500C targets Mouse GM-CSF in ELISA, and WB applications and shows reactivity with mouse samples.
The MM500C immunogen is recombinant mouse GM-CSF.
MM500C detects Mouse GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The MM500C mouse GMCSF antibody (clone 22E9) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM500DB (biotinylated conjugate of clone 31G6) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody MM500C (clone 22E9) and biotinylated antibody MM500DB (clone 31G6) have successfully been used in combination with recombinant mouse GMCSF protein SMGMCSF in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Colony-stimulating factor; CSF; GM-CSF; granulocyte-macrophage colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; Granulocyte-macrophage colony-stimulating factor; molgramostin; put. GM-CSF; sargramostim
Gene Aliases: Csf2; Csfgm; Gm-CSf; GMCSF; MGI-IGM
UniProt ID: (Mouse) P01587
Entrez Gene ID: (Mouse) 12981