M500A targets Human GM-CSF in ELISA, and WB applications and shows reactivity with Human samples.
The M500A immunogen is glycosylated, CHO-derived recombinant human GM-CSF.
M500A detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The M500A GMCSF antibody (clone 3092) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M501B (biotinylated conjugate of clone 1089). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody M500A (clone 3092) and biotinylated antibody M501B (clone 1089) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor; Granulocyte-macrophage colony-stimulating factor; Molgramostin; Sargramostim
Gene Aliases: CSF2; GMCSF
UniProt ID: (Human) P04141
Entrez Gene ID: (Human) 1437