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|Tested species reactivity||Human|
|Published species reactivity||Bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-20 µg/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-077 detects GM130 from human samples.
PA1-077 has been successfully used in Western blot, flow cytometry, and immunofluorescence procedures. By Western blot, PA1-077 detects a ~135 kDa band representing GM130 from Hela cell lysate.
The PA1-077 immunogen is a synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130.
GM130 is a putative cis-Golgi matrix protein that belongs to the golgin family of autoantigens possessing extensive coiled-coil domains. This protein interacts with GRASP65 and acts as a receptor for the vesicle docking protein p115 during vesicle fusion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hyaluronan synthesis mediates the fibrotic response of keratocytes to transforming growth factor beta.
PA1-077 was used in western blot to investigate the effect of transforming growth factor beta on keratocytes and its mechanism
|Guo N,Li X,Mann MM,Funderburgh ML,Du Y,Funderburgh JL||The Journal of biological chemistry (285:32012)||2010|
130 kDa cis-Golgi matrix protein; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; golgin-95; SY11 protein