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Invitrogen
GNA13 Polyclonal Antibody
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FIGURE: 1 / 3
GNA13 Antibody (PA5-100664) in ICC/IF
Immunofluorescent analysis of GNA13 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GNA13 polyclonal antibody (Product # PA5-100664) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100664
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GNA13(Accession Q14344), corresponding to amino acid residues V10-G60.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GNA13.
Target Information
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:15240885, PubMed:16787920, PubMed:16705036, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866). GNA13-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway (PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: G alpha 13; G alpha-13; G-protein subunit alpha-13; Galpha 13; guanine nucleotide binding protein (G protein), alpha 13; guanine nucleotide binding protein, alpha 13; Guanine nucleotide-binding protein subunit alpha-13
Gene Aliases: AU024132; AU043124; G13; Galpha13; Gna-13; GNA13
UniProt ID: (Human) Q14344, (Mouse) P27601, (Rat) Q6Q7Y5
Entrez Gene ID: (Human) 10672, (Mouse) 14674, (Rat) 303634
GTPase activity
signal transducer activity
protein binding
GTP binding
G-protein beta/gamma-subunit complex binding
type 1 angiotensin receptor binding
D5 dopamine receptor binding
metal ion binding
nucleotide binding
G-protein coupled receptor binding
guanyl nucleotide binding
heterotrimeric G-protein
patterning of blood vessels
in utero embryonic development
movement of cell or subcellular component
signal transduction
adenylate cyclase-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
Rho protein signal transduction
regulation of cell shape
cell differentiation
platelet activation
regulation of cell migration
activation of phospholipase D activity
angiogenesis
G-protein coupled receptor signaling pathway
small GTPase mediated signal transduction
intracellular signal transduction
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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