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Invitrogen
GPI Polyclonal Antibody
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FIGURE: 1 / 10
GPI Antibody (PA5-29665) in ICC/IF
Immunofluorescence analysis of GPI was performed using 70% confluent log phase HeLa cells. The cells were fixed with ice cold acetone for 5 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GPI Polyclonal Antibody (Product # PA5-29665) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (... View More
Product Details
PA5-29665
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human GPI. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: H1299, Raji.
Predicted reactivity: Rat (89%), Xenopus laevis (81%), Pig (92%), Rabbit (92%), Chicken (86%), Chimpanzee (99%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AMF; Autocrine motility factor; D-hexose-6-phosphate anomerase; Glucose-6-phosphate C2-epimerase; Glucose-6-phosphate isomerase; glucose-6-phosphate isomerase {ECO:0000250|UniProtKB:P06745}; GPI; GPI {ECO:0000250|UniProtKB:P06745}; gpi {ECO:0000312|RGD:2727}; hexose monophosphate isomerase; hexosephosphate isomerase; Neuroleukin; neuroleukin {ECO:0000250|UniProtKB:P06745}; NLK; NLK {ECO:0000250|UniProtKB:P06745}; oxoisomerase; PGI; PGI {ECO:0000250|UniProtKB:P06745}; PHI; PHI {ECO:0000250|UniProtKB:P06745}; Phosphoglucose isomerase; phosphoglucose isomerase {ECO:0000250|UniProtKB:P06745}; phosphohexomutase; Phosphohexose isomerase; phosphohexose isomerase {ECO:0000250|UniProtKB:P06745}; phosphosaccharomutase; SA-36; sperm antigen; Sperm antigen 36; sperm antigen-36; unnamed protein product
Gene Aliases: AMF; CNSHA4; GNPI; GPI; Gpi1; NLK; PGI; PHI; SA-36; SA36
UniProt ID: (Human) P06744, (Rat) Q6P6V0
Entrez Gene ID: (Human) 2821, (Rat) 292804
in utero embryonic development
mesoderm formation
positive regulation of immunoglobulin production
carbohydrate metabolic process
fructose 6-phosphate metabolic process
gluconeogenesis
glycolytic process
humoral immune response
signal transduction
hemostasis
learning or memory
positive regulation of endothelial cell migration
response to estradiol
response to progesterone
response to testosterone
erythrocyte homeostasis
response to immobilization stress
response to muscle stretch
glucose homeostasis
negative regulation of apoptotic process
response to cadmium ion
glucose 6-phosphate metabolic process
canonical glycolysis
carbohydrate derivative metabolic process
angiogenesis
methylglyoxal biosynthetic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of neuron apoptotic process
aldehyde catabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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