Invitrogen

GPR177 Polyclonal Antibody

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GPR177 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of GPR177 in paraffin embedded human adrenal gland tissue using a GPR177 polyclonal antibody (Product # PA5-98483) at a dilution of 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

GPR177 Antibody in Western Blot (WB) — Western Blot analysis of GPR177 using a GPR177 Polyclonal antibody (Product # PA5-98483) at a concentration of 5.4 µg/mL. Positive WB detected in: HepG2 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 52 kDa.

Product Details

PA5-98483

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Protein wntless homolog protein (123-232AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.73 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2813096

Target Information

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EVI; G protein-coupled receptor 177; Integral membrane protein GPR177; Protein evenness interrupted homolog; Protein wntless homolog; Putative NF-kappa-B-activating protein 373; putative NFkB activating protein 373

Gene Aliases: C1orf139; EVI; GPR177; mig-14; MRP; UNQ85/PRO18667; WLS

UniProt ID: (Human) Q5T9L3

Entrez Gene ID: (Human) 79971

Function(s)