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Invitrogen
GRAP2 Polyclonal Antibody
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FIGURE: 1 / 1
GRAP2 Antibody (PA5-97154) in WB
Western blot analysis of GRAP2 in extracts of Jurkat cells. Samples were incubated with GRAP2 Polyclonal antibody (Product # PA5-97154) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Product Details
PA5-97154
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human GRAP2 (NP_0048011)
Conjugate
Form
Liquid
Concentration
2.76 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MEAVAKFDFT ASGEDELSFH TGDVLKILSN QEEWFKAELG SQEGYVPKNF IDIQFPKWFH EGLSRHQAEN LLMGKEVGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD NKGNYFLWTE KFPSLNKLVD YYRTNSISRQ KQIFLRDRTR EDQGHRGNSL DRRSQGGPHL SGAVGEEIRP SMNRKLSDHP PTLPLQQHQH QPQPPQYAPA PQQLQQPPQQ RYLQHHHFHQ ERRGGSLDIN DGHCGTGLGS EMNAALMHRR HTDPVQLQAA GRVRWARALY DFEALEDDEL GFHSGEVVEV LDSSNPSWWT GRLHNKLGLF PANYVAPMTR; Positive Samples: Jurkat; Cellular Location: Cytoplasm, Endosome, Nucleus
Target Information
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adapter protein GRID; GADS protein; GRB-2-like protein; GRB-2-related monocytic adapter protein; GRB2-related adapter protein 2; Grb2-related adaptor downstream of Sch; GRB2-related adaptor protein 2; GRB2-related protein with insert domain; GRB2L; GRBLG; GRBX; Grf-40; Grf40 adapter protein; Growth factor receptor-binding protein; growth factor receptor-bound protein 2-related adaptor protein 2; Hematopoietic cell-associated adapter protein GrpL; Hematopoietic cell-associated adaptor protein GrpL; MONA; monocytic adapter; monocytic adaptor; P38; Protein GADS; RP3-370M22.1; SH3-SH2-SH3 adapter Mona; SH3-SH2-SH3 adaptor molecule
Gene Aliases: GADS; GRAP-2; GRAP2; GRB2L; GRBLG; GrbX; Grf40; GRID; GRPL; Mona; P38
UniProt ID: (Human) O75791, (Mouse) O89100
Entrez Gene ID: (Human) 9402, (Mouse) 17444
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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