Search
Invitrogen
Granzyme B Monoclonal Antibody (GB11), PE-Texas Red
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 6
Granzyme B Antibody (GRB17) in Flow
Nature communications 2022 -
Fig. 2 FTY720 treatment in the first weeks of ART retains cytolytic cells but does not enhance their functionality in the LN. a Frequency of CD4+ T cells, b CD8+ T cells, and ( c ) ratio of CD4+/CD8+ T cells in lymph node (LN) mononuclear cells at baseline (week -1) and at 8 weeks of treatment in early FTY720 ( n = 8 RMs) or control (n = 14 RMs) SIV-infected RMs. d Frequency of LN perforin+ (Perf+), e granz... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
GRB17
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GB11
Immunogen
Human Granzyme B.
Conjugate
PE-Texas Red
PE-Texas Red
PE-Texas Red
Excitation/Emission Max
567/615 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
According to the literature this antibody recognizes the serine protease, granzyme B1. This enzyme is involved in apoptotic cell death. Granzyme B, as well as other enzymes contained in lytic granules, is released primarily by cytotoxic T lymphocytes (CTLs). In addition to immunostaining for flow cytometry, the GB11 mAb has been shown to be useful as an ELISA capture antibody.
Target Information
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: alternatively spliced; cathepsin G-like 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; fragmentin 2; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); GranzymeB; human lymphocyte protein; Human lymphocyte protein (Hlp); OTTHUMP00000028189; proteolytic serine esterase; serine protease; serine protease-like protein precursor; T-cell serine protease 1-3E
Gene Aliases: C11; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT
UniProt ID: (Human) P10144
Entrez Gene ID: (Human) 3002
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support