Invitrogen

GRK1 Polyclonal Antibody

1 Published Figure

1 Reference

View all (16) GRK1 antibodies

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Questions & Answers

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GRK1 Antibody in Western Blot (WB) — Western blot analysis of GRK1 using a GRK1 polyclonal antibody (Product # PA5-13725) in HeLa cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).

GRK1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a GRK1 polyclonal antibody (Product # PA5-13725), followed by HRP-conjugated secondary antibody and DAB staining.

GRK1 Antibody — Fig. 5 Intravitreous injection of sunitinib MPs in mice with type 3 CNV reduces photoreceptor cell death. Rho/VEGF mice were given an intravitreous injection of 10 ug of sunitinib microparticles (Suni MP) in one eye and 10 ug of empty MP in the other eye or 40 ug of aflibercept in one eye and PBS in the fellow eye. At P42, mice ( n = 7 for each group) were euthanized and serial frozen ocular sections were cut from the superior pole of the eye (S0) to the inferior pole (I0) and sections 25% (S1 and I1), 50% (S2 and I2), and 75% (S3 and I3) of the distance between each pole and the optic nerve (ON) were stained with hematoxylin and outer nuclear layer (ONL) thickness was measured by image analysis by a masked investigator. The ONL of sections from the S2 location of Suni MP-injected eyes appeared thicker than those from empty MP-injected eyes, but those from aflibercept-injected eyes appeared similar to those from PBS-injected eyes ( a scale bar = 100 um). The mean (+-SEM) ONL thickness was significantly greater at three of six locations in Suni MP-injected eyes compared with empty MP-injected eyes, but there was no difference between aflibercept- and PBS-injected eyes ( b ). At P49, mice ( n = 5 for each group) were euthanized and immunoblots of retinal homogenates from Suni MP-injected eyes showed prominent bands for rhodopsin kinase (GRK-1) ( c ). Denistometry showed that the mean (+-SEM) GRK-1/Actin ratio was significantly greater in retinas from Suni MP-injected eyes compa

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human GRK1

Conjugate

Unconjugated

Form

Liquid

Concentration

Lot-specific

Purification

Size-exclusion - Dialysis, Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2115610

Target Information

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.