Immunofluorescent analysis of Grp78/BiP (green) in MDCK cells. The cells were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a Grp78/BiP rabbit polyclonal antibody (Product # PA1-014A), at a concentration of 10ug/ml in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 30 minutes at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Rat, Hamster, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP78.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-014A detects glucose regulated protein/BiP (GRP78) human, rat, mouse, and canine samples.
PA1-014A has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 78 kDa protein representing GRP78 from rat liver lysates.
The PA1-014A immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP78.
Reconstitute with PBS.
The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degredation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PERK regulated miR-424(322)-503 cluster fine-tunes activation of IRE1 and ATF6 during Unfolded Protein Response.
PA1-014A was used in western blot to study the miR-424(322)-503 cluster in the unfolded protein response
|Gupta A,Hossain MM,Read DE,Hetz C,Samali A,Gupta S||Scientific reports (5:null)||2015|
Proteomic analysis of ¿-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor complexes.
PA1-014A was used in western blot to identify novel components of brain AMPA receptors using a proteomic approach
|Kang MG,Nuriya M,Guo Y,Martindale KD,Lee DZ,Huganir RL||The Journal of biological chemistry (287:28632)||2012|
Ras inhibits endoplasmic reticulum stress in human cancer cells with amplified Myc.
PA1-014A was used in western blot to study the effect of Ras on endoplasmic reticulum stress in human cancer cells and its mechanism
|Yaari-Stark S,Shaked M,Nevo-Caspi Y,Jacob-Hircsh J,Shamir R,Rechavi G,Kloog Y||International journal of cancer (126:2268)||2010|
Dysferlin overexpression in skeletal muscle produces a progressive myopathy.
PA1-014A was used in western blot to characterize the effect of dysferlin level on myopathy
|Glover LE,Newton K,Krishnan G,Bronson R,Boyle A,Krivickas LS,Brown RH||Annals of neurology (67:384)||2010|
HtrA2 regulates beta-amyloid precursor protein (APP) metabolism through endoplasmic reticulum-associated degradation.
PA1-014A was used in western blot to study the effect of HtrA2 on beta amyloid precursor protein metabolism.
|Huttunen HJ,Guénette SY,Peach C,Greco C,Xia W,Kim DY,Barren C,Tanzi RE,Kovacs DM||The Journal of biological chemistry (282:28285)||2007|
Identification of mRNA/protein (mRNP) complexes containing Puralpha, mStaufen, fragile X protein, and myosin Va and their association with rough endoplasmic reticulum equipped with a kinesin motor.
PA1-014A was used in western blot to investigate the function of the rough endoplasmic reticulum structure during the transport of polyribosomes.
|Ohashi S,Koike K,Omori A,Ichinose S,Ohara S,Kobayashi S,Sato TA,Anzai K||The Journal of biological chemistry (277:37804)||2002|
Response of neurons to an irreversible inhibition of endoplasmic reticulum Ca(2+)-ATPase: relationship between global protein synthesis and expression and translation of individual genes.
PA1-014A was used in western blot to investigate Tg-induced inhibition and recovery of protein synthesis and corresponding changes of particular genes in mRNA level and protein level
|Mengesdorf T,Althausen S,Oberndorfer I,Paschen W||The Biochemical journal (356:805)||2001|
Developmental regulation of FKBP65. An ER-localized extracellular matrix binding-protein.
PA1-014A was used in western blot to investigate the regulation of FKBP65 subcellular localization and expression level during organism development.
|Patterson CE,Schaub T,Coleman EJ,Davis EC||Molecular biology of the cell (11:3925)||2000|
Membrane topology of the murine fatty acid transport protein 1.
PA1-014A was used in immunocytochemistry to study the topology of murine fatty acid transport protein
|Lewis SE,Listenberger LL,Ory DS,Schaffer JE||The Journal of biological chemistry (276:37042)||2001|
Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), localizes to the basolateral plasma membrane of the retinal pigment epithelium.
PA1-014A was used in immunoprecipitation to investigate the localization of bestrophin in rat retinal pigment epithelium.
|Marmorstein AD,Marmorstein LY,Rayborn M,Wang X,Hollyfield JG,Petrukhin K||Proceedings of the National Academy of Sciences of the United States of America (97:12758)||2000|