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          • Primary Antibodies ›
          • GST Antibodies

          Invitrogen

          GST Tag Polyclonal Antibody

          5 Published Figures
          16 References
          View all (91) GST antibodies

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          Cite GST Tag Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          • Published Figures (5)
          GST Tag Antibody in Western Blot (WB)
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          GST Tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          GST Tag Antibody (CAB4169) in WB

          Western blot analysis was performed on recombinant proteins (1 µg) of Neurophilin 2 (Lane 1), DOT1 (Lane 2) and GST Tag (Lane 3). The blots were probed with Anti-GST Tag Rabbit Polyclonal Antibody (Product # CAB4169, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 26 kDa band corresponding to GST Tag was observed while different GST tagged proteins showed their corresponding molecular weights. Known quantity of protein samples were ele... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Immunoprecipitation (IP)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot, Immunoprecipitation (WB, IP)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)
          GST Tag Antibody in Western Blot (WB)

          Product Details

          CAB4169

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5-2 µg/mL
          View 11 publications 11 publications

          Immunoprecipitation (IP)

          Assay-dependent
          View 2 publications 2 publications

          Peptide Array (Array)

          -
          View 1 publication 1 publication

          GST Pull Down (GST-PD)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Purified recombinant GST.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          BBS, pH 8.4

          Contains

          no preservative

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10709998

          Product Specific Information

          CAB4169 detects GST in samples.

          CAB4169 has been successfully used in Western Blot applications.

          The CAB4169 immunogen is purified recombinant GST.

          Target Information

          GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

          View more View less

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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