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Western blot analysis was performed on recombinant proteins (1 ug) of Neurophilin 2 (Lane 1), DOT1 (Lane 2) and GST Tag (Lane 3). The blots were probed with Anti-GST Tag Rabbit Polyclonal Antibody (Product# CAB4169, 0.5-2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G21234,1:5000 dilution). A 26 kDa band corresponding to GST Tag was observed while different GST tagged proteins showed their corresponding molecular weights. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant GST.|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.4|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CAB4169 detects GST in samples.
CAB4169 has been successfully used in Western Blot applications.
The CAB4169 immunogen is purified recombinant GST.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cool-1/βPIX functions as a guanine nucleotide exchange factor in the cycling of Cdc42 to regulate insulin secretion.
CAB4169 was used in immunoprecipitation to identify the interacting partners of Cdc42 and their combined effects on insulin release
|Kepner EM,Yoder SM,Oh E,Kalwat MA,Wang Z,Quilliam LA,Thurmond DC||American journal of physiology. Endocrinology and metabolism (301:E1072)||2011|
Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.
CAB4169 was used in western blot to investigate the effect of syntaxin 4 S-nitrosylation on insulin granule exocytosis
|Wiseman DA,Kalwat MA,Thurmond DC||The Journal of biological chemistry (286:16344)||2011|
Glutathione-S-Transferase; GST protein; GST Tag