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          • Primary Antibodies ›
          • GUSB Antibodies

          Invitrogen

          GUSB Recombinant Rabbit Monoclonal Antibody (4H2)

          View all (16) GUSB antibodies

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          Cite GUSB Recombinant Rabbit Monoclonal Antibody (4H2)

          Additional Information:
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          • Antibody Testing Data (1)
          GUSB Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          GUSB Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          GUSB Antibody (MA573201) in Flow

          Flow cytometry analysis of GUSB in HelpG2 cells were fixed with 4% PFA (10 min at room temp) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temp). Followed by secondary antibody incubation for 40 min at room temperature. Samples were incubated using GUSB Recombinant Monoclonal (Product # MA573201) at dilution 1:100 (green). Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          GUSB Antibody in Flow Cytometry (Flow)

          Product Details

          MA573201

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Flow Cytometry (Flow)

          1:50-1:100
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          4H2

          Immunogen

          KLH conjugated synthetic peptide derived from human beta Glucuronidase.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.75 mg/mL

          Amount

          75 µg

          Purification

          Protein A

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It is a tetrameric glycoprotein composed of identical subunits. The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: beta glucuronidase; beta-D-glucuronidase; Beta-G1; Beta-glucuronidase; beta-glucuronidase precursor (EC 3.2.1.31); unnamed protein product

          View more View less

          Gene Aliases: BG; GUSB; MPS7

          View more View less

          UniProt ID: (Human) P08236

          View more View less

          Entrez Gene ID: (Human) 2990

          View more View less

          Function(s)
          hydrolase activity, hydrolyzing O-glycosyl compounds beta-glucuronidase activity receptor binding hydrolase activity hydrolase activity, acting on glycosyl bonds protein domain specific binding carbohydrate binding
          Process(es)
          carbohydrate metabolic process glycosaminoglycan catabolic process heparan sulfate proteoglycan catabolic process chondroitin sulfate catabolic process hyaluronan catabolic process
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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