Invitrogen

Gelsolin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (21) Gelsolin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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Gelsolin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin (Product # PA5-27350) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Gelsolin Antibody in Western Blot (WB) — Gelsolin Polyclonal Antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin Polyclonal Antibody (Product # PA5-27350) diluted at 1:1,000.

Gelsolin Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. HeLa cells were transfected with Gelsolin shRNA and decrease in signal intensity was observed in Western blot application using Gelsolin Polyclonal Antibody (Product # PA5-27350). {KD}

Product Details

PA5-27350

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 561 and 782 of Human Gelsolin View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2544826

Product Specific Information

Recommended positive controls: H1299, HeLa, human plasma, rat plasma, mouse plasma.

Predicted reactivity: Mouse (94%), Rat (94%), Chicken (81%), Sheep (98%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Renal carcinoma/kidney progenitor cell chimera organoid as a novel tumorigenesis gene discovery model.

Disease models & mechanisms

Xu Q,Junttila S,Scherer A,Giri KR,Kivelä O,Skovorodkin I,Röning J,Quaggin SE,Marti HP,Shan J,Samoylenko A,Vainio SJ

PA5-27350 was used in Western Blotting to compare renal ontogenesis control genes to the genes involved in kidney cancer which may provide new growth-associated gene screens in the context of the behavioural roles of cancer cells.