Invitrogen

GluR1 Monoclonal Antibody (S355-1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Protocols

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GluR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GluR1 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GluR1 monoclonal antibody (Product # MA5-27694) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GluA1/GluR1 Antibody, D) Merged image. Magnification: 60x.

GluR1 Antibody in Western Blot (WB) — Western blot was performed using Anti-GluR1 Monoclonal Antibody (S355-1) (Product # MA5-27694) and a 100 kDa band corresponding to GluR1 was observed in brain tissues. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Liver (Lane 2), Rat Brain (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GluR1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of GluR1 was observed in Mouse Brain, Rat Brain in comparison to Mouse Liver and Rat Liver using Anti-GluR1 Monoclonal Antibody (S355-1) (Product # MA5-27694) in Western Blot. {RE}

Product Details

MA5-27694

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:100	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	S355-1
Immunogen	Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2735203

Target Information

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AI853806; AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluR-1; GluR-A; GluR-K1; GluR1; GluRK1; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GPRC1A; Gria 1; GRM1A; HGBR1; HIPA; MGC13325; MGC133252; mGlu1; MGLUR1; MGLUR1A; NMDA1; NMDAR1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; RP11-350O14.1

Gene Aliases: 2900051M01Rik; Glr-1; Glr1; GluA1; GLUH1; Glur-1; GluR-A; gluR-K1; GLUR1; GLURA; GRIA1; HBGR1; HIPA1

UniProt ID: (Human) P42261, (Rat) P19490, (Mouse) P23818

Entrez Gene ID: (Human) 2890, (Rat) 50592, (Mouse) 14799

Molecular Function: transmembrane signal receptor