|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal region of rat GLRA1 conjugated to KLH|
|Storage buffer||5mM Ammonium bicarbonate, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This antibody is specific for the ~48 kDa alpha 1 and alpha 2 subunits of the Glycine Receptor in Western blots of rat spinal cord and brain stem and in cell extracts, and does not recognize other Glycine Receptor subunits. Immunolabeling is blocked by preadsorption of antibody with the peptide immunogen.
Reconstitute with 100 ul PBS.
The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor. The receptor mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Role of an Absolutely Conserved Tryptophan Pair in the Extracellular Domain of Cys-Loop Receptors.
PA1-4661 was used in immunohistochemistry to analyze the extracellular domain of cys-loop receptors and the role of an absolutely conserved tryptophan pair
|Braun N,Lynagh T,Yu R,Biggin PC,Pless SA||ACS chemical neuroscience (7:339)||2016|