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Invitrogen
F(ab)-Goat anti-Rabbit IgG Fc Secondary Antibody, Alexa Fluor™ 555
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FIGURE: 1 / 6
Rabbit IgG Fc Secondary Antibody (A66786) in ICC/IF
Immunofluorescence analysis of F(ab)-Goat anti-Rabbit IgG Fc Secondary Antibody, Alexa Fluor™ 555 (Product # A66786) was performed on fixed and permeabilized HeLa cells blocked with 2% BSA at room temperature and stained with TOMM20 Polyclonal Antibody (Product # PA5-52843; used at 0.2 µg/mL) in 0.1% BSA overnight at 4-degree Celsius. This was detected by incubating with F(ab)-Goat anti-Rabbit IgG Fc Second... View More
Product Details
A66786
Applications
Tested Dilution
Western Blot (WB)
0.2-0.4 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
1 µg/mL
Immunocytochemistry (ICC/IF)
0.5-2 µg/mL
Flow Cytometry (Flow)
0.5-2 µg/mL
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG, F(ab)
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Rabbit IgG Fc
Conjugate
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG Fc
Antibody Form
F(ab) Fragment
Product Specific Information
This F(ab)-Goat anti-Rabbit IgG Fc specific antibody product is a goat polyclonal antibody F(ab) fragment that is specific to the Fc region of rabbit IgG. The product has minimal to no cross-reactivity towards polyclonal mouse, rat, guinea pig, bovine, hamster, horse, sheep, human, monkey, donkey IgG, and chicken IgY as tested by direct ELISA. Cross-reactivity towards other immunoglobulins has not been tested. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
F(ab) antibody fragment offer several benefits over whole IgGs, due to their smaller size (~50 kD vs. ~150 kD) and the absence of the Fc region. The smaller size is expected to allow better penetration into tissue sections and improved staining in applications such as IHC. The lack of Fc region results in reduced nonspecific background staining when staining cells/ tissues with Fc receptors (such as immune cells) and other Fc interaction-related interference. Fc specific F(ab) fragments provide a range of multiplexing options beyond what is possible with whole IgGs for applications such as immunocytochemistry, immunohistochemistry, flow cytometry, and western blotting.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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