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Invitrogen
Goat anti-Rabbit IgG Fc Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 488
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FIGURE: 1 / 3
Rabbit IgG Fc Secondary Antibody (SA5-10378-AFP488) in ICC/IF
Immunofluorescence analysis of Goat anti-Rabbit IgG Fc Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # SA5-10378-AFP488) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer (Product # 37515) at room temperature and stained with H3K4me3 Recombinant Rabbit Monoclonal Antibody (14H24L5) (Product # 703849, 1:250) in SuperBlock™ Blocking Buff... View More
Product Details
SA5-10378-AFP488
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
1:250
Immunocytochemistry (ICC/IF)
1:1,000
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Purified Rabbit IgG
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Target
IgG Fc
Antibody Form
Whole Antibody
Product Specific Information
The Goat anti-Rabbit IgG Fc Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 is specific to Fc region of Rabbit IgG with moderate cross reactivity to Monkey IgG. It showed no cross-reactivity to any other immunoglobulins tested from other species.
The sensitivity and specificity of each lot is confirmed using ELISA.
Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency.
Alexa Fluor™ Plus dyes have been chemically optimized to provide higher signal to noise ratios and minimized cross-reactivity to help detect low-abundance targets in rare or precious samples.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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