Immunofluorescence analysis of Goat anti-Rat IgG (H+L) Secondary Antibody, Cy5® was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2µg/ml Rat primary antibody for 3 hours at room temperature. Goat anti-Rat IgG (H+L) Secondary Antibody, Cy5® (Product # A10525) was used at a concentration of 4µg/ml in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||4 µg/ml|
|Immunofluorescence (IF)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||10 µg/ml||
Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model.
A10525 was used in immunohistochemistry - frozen section to develop an in vivo approach using intracerebral grafting of mesenchymal stem cells to secrete interleukin 13
|Le Blon D,Guglielmetti C,Hoornaert C,Quarta A,Daans J,Dooley D,Lemmens E,Praet J,De Vocht N,Reekmans K,Santermans E,Hens N,Goossens H,Verhoye M,Van der Linden A,Berneman Z,Hendrix S,Ponsaerts P||Journal of neuroinflammation (13:null)||2016|
Lineage tracing of cells involved in atherosclerosis.
A10525 was used in immunohistochemistry - frozen section to identify the origin of vascular smooth muscle cells and macrophages within atherosclerosis lesions
|Albarrán-Juárez J,Kaur H,Grimm M,Offermanns S,Wettschureck N||Atherosclerosis (251:445)||2016|
Impermanence of dendritic spines in live adult CA1 hippocampus.
A10525 was used in immunohistochemistry to test if hippocampal synapse lifetime matches the longevity of hippocampal memory
|Attardo A,Fitzgerald JE,Schnitzer MJ||Nature (523:592)||2015|
|Not Applicable||Not Cited||
Native elongating transcript sequencing reveals human transcriptional activity at nucleotide resolution.
A10525 was used in western blot to use native elongating transcript sequencing in human cells to globally map strand-specific RNA polymerase II density at nucleotide resolution
|Mayer A,di Iulio J,Maleri S,Eser U,Vierstra J,Reynolds A,Sandstrom R,Stamatoyannopoulos JA,Churchman LS||Cell (161:541)||2015|