|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Rat Mu immunonglobin|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse IgG, mouse serum, and human serum prior to conjucation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Goat anti-rat IgM antibodies have been purified by rat IgM-affinity chromatography and react with the mu chain of rat IgM, as determined by ELISA. The rat anti-IgM may also react with IgM from other species.
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human skin cells that express stage-specific embryonic antigen 3 associate with dermal tissue regeneration.
A-21212 was used in immunocytochemistry to isolate and characterize SSEA3-expressing regeneration-associated cells
|Vega Crespo A,Awe JP,Reijo Pera R,Byrne JA||BioResearch open access (1:25)||2012|
|Not Applicable||Not Cited||Generation of keratinocytes from normal and recessive dystrophic epidermolysis bullosa-induced pluripotent stem cells.||Itoh M,Kiuru M,Cairo MS,Christiano AM||Proceedings of the National Academy of Sciences of the United States of America (108:8797)||2011|
|Not Applicable||Not Cited||Human intestinal tissue and cultured colonic cells contain globotriaosylceramide synthase mRNA and the alternate Shiga toxin receptor globotetraosylceramide.||Zumbrun SD,Hanson L,Sinclair JF,Freedy J,Melton-Celsa AR,Rodriguez-Canales J,Hanson JC,O'Brien AD||Infection and immunity (78:4488)||2010|
|Not Applicable||Not Cited||Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.||Oda Y,Yoshimura Y,Ohnishi H,Tadokoro M,Katsube Y,Sasao M,Kubo Y,Hattori K,Saito S,Horimoto K,Yuba S,Ohgushi H||The Journal of biological chemistry (285:29270)||2010|
|Not Applicable||Not Cited||Laminin isoforms of lymph nodes and predominant role of alpha5-laminin(s) in adhesion and migration of blood lymphocytes.||Gorfu G,Virtanen I,Hukkanen M,Lehto VP,Rousselle P,Kenne E,Lindbom L,Kramer R,Tryggvason K,Patarroyo M||Journal of leukocyte biology (84:701)||2008|
|Not Applicable||Not Cited||Beta-1,2- and alpha-1,2-linked oligomannosides mediate adherence of Candida albicans blastospores to human enterocytes in vitro.||Dalle F,Jouault T,Trinel PA,Esnault J,Mallet JM,d'Athis P,Poulain D,Bonnin A||Infection and immunity (71:7061)||2003|