|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human GM-CSF|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5 - 2.0 ug/ml|
|Functional Assay (FN)||0.3-0.5 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||0.1 - 0.2ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections.
Reconstitute with 0.1 ml of distilled water. For long term storage 0.09% sodium azide is recommended. After reconstitution, store at -20°C.
This antibody may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with PA1-84988 as the detection antibody.
GM-CSF (granulocyte-macrophage colony-stimulating factor) is a 14.6kDa haematopoietic growth factor which exists in both glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The ability of recombinant GM-CSF to increase haematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.