|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues E(20) I I G G H E A K P H S R P Y M A Y L(38) of human Granzyme B.|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26616 detects Granzyme B from human, rat, and mouse samples.
PA1-26616 has been successfully used in Immunofluorescence, immunohistochemistry (frozen), and immunohistochemistry (paraffin) procedures. For IHC (paraffin) procedures perform heat mediated antigen retrieval using 10mM citrate buffer (p.H. 6.0) before commencing with the IHC staining protocol.
Positive Control: Tonsil
The PA1-26616 immunogen is a synthetic peptide corresponding to residues E(20) I I G G H E A K P H S R P Y M A Y L(38) of human Granzyme B.
Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned. Human granzymes B is involved in target cell apoptosis during lymphocyte-mediated cytotoxicity. This antibody is useful in the localization of granzyme B containing lytic granules and for the characterization of activated CTLs or NK cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.