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Invitrogen
HA Tag Polyclonal Antibody
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FIGURE: 1 / 6
HA Tag Antibody (PA1-985) in WB
Western blot was performed using Anti-HA Tag Polyclonal Antibody (Product # PA1-985) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3, 40 µg (Lane 3), HA-H3, 20 µg (Lane 4), HA-H3, 10 µg (Lane 5), HA-H3, 5 µg (Lane 6), p65-HA, 40 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8 and 25 ng Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred ont... View More
Product Details
PA1-985
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the residues Y P Y D V P D Y A of the HA epitope of influenza hemagglutinin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-985 detects HA tagged proteins. This antibody is specific for the HA tag and does not detect other proteins.
PA1-985 has been successfully used in Western blot procedures. By Western blot, this antibody detects the presence of HA-tagged proteins.
The PA1-985 immunogen is a synthetic peptide corresponding to the residues Y P Y D V P D Y A of the HA epitope of influenza hemagglutinin.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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