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HAND1 Antibody (720261) in WB
Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1) and Rat Heart (Lane 2). The blots were probed with Anti-HAND1 Rabbit Polyclonal antibody (Product # 720261, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 23 kDa band corresponding to HAND1 was observed specifically in heart tissue lysates. Known quantity of protein samples were electrophoresed using Novex® NuPA... View More
Product Details
720261
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to Human HAND1 (aa 94-105, 173-188)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633222
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Bovine and Rabbit
Target Information
HAND1 belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHa27; Class A basic helix-loop-helix protein 27; eHAND; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; heart and neural crest derivatives expressed transcript 1; Heart- and neural crest derivatives-expressed protein 1; Helix-loop-helix transcription factor expressed in extraembryonic mesoderm and trophoblast; Th1; Thing-1
Gene Aliases: BHLHA27; EHAND; Ehand1; HAND1; Hxt; Th1; Thing1
UniProt ID: (Human) O96004, (Mouse) Q64279, (Rat) P97832
Entrez Gene ID: (Human) 9421, (Mouse) 15110, (Rat) 59112
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator activity
transcription corepressor activity
protein binding
transcription factor binding
enzyme binding
identical protein binding
protein homodimerization activity
bHLH transcription factor binding
transcription regulatory region DNA binding
protein heterodimerization activity
DNA binding
sequence-specific DNA binding
protein dimerization activity
molecular_function
basic helix-loop-helix transcription factor
immunoglobulin receptor superfamily
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
mesoderm formation
blastocyst development
trophectodermal cell differentiation
heart looping
embryonic heart tube formation
cardiac left ventricle formation
cardiac right ventricle formation
transcription from RNA polymerase II promoter
heart development
embryonic heart tube development
odontogenesis of dentin-containing tooth
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
ventricular cardiac muscle tissue morphogenesis
cardiac septum morphogenesis
mesenchyme development
cartilage morphogenesis
trophoblast giant cell differentiation
negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding
in utero embryonic development
cell fate determination
heart morphogenesis
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
positive regulation of transcription, DNA-templated
determination of heart left/right asymmetry
biological_process
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