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HAS3 Antibody (PA5-93099) in WB
Western blot analysis of HAS3 in extracts of various cell lines. Samples were incubated with HAS3 Polyclonal antibody (Product # PA5-93099) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-93099
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 67-281 of human HAS3 (NP_6195151)
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806623
Product Specific Information
Immunogen sequence: HRRMRRAGQA LKLPSPRRGS VALCIAAYQE DPDYLRKCLR SAQRISFPDL KVVMVVDGNR QEDAYMLDIF HEVLGGTEQA GFFVWRSNFH EAGEGETEAS LQEGMDRVRD VVRASTFSCI MQKWGGKREV MYTAFKALGD SVDYIQVCDS DTVLDPACTI EMLRVLEEDP QVGGVGGDVQ PPGKGMAVED DQVQAAQVRA TEAWSVHQRH VSREQ; Positive Samples: A-549, HL-60, SKOV3, SW480, Mouse heart, Rat liver, Rat heart; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
HAS1, HAS2 and HAS3 are HA (hyaluronan or hyaluronic acid) synthase proteins. The extracellular matrix in most vertebrates express HA, which is a high molecular weight linear polysaccharide composed of alternating glucuronic acid and N-acetylglucosamine residues linked by b-1,3 and b-1,4 glycosidic bonds. The three HAS genes show distinct patterns of expression during development and their protein products play significantly different roles in the formation of the HA matrix. Both HAS1 and HAS2 synthesize high molecular-weight HA, whereas HAS3 produces lower molecular weight HA. The expression of the three HAS isoforms is more prominent in growing cells than in resting cells and is differentially regulated by various stimuli, suggesting distinct functional roles of the three proteins. HAS3 produces both secreted and cell-associated forms of hyaluronan and is the most active of the three isoforms of this enzyme in adults. HAS3 gene expression plays a crucial role in the regulation of hyaluronan synthesis in the epidermis. Specifically, IFN-g markedly upregulates HAS3 mRNA, whereas TGFbeta downregulates HAS3 transcript levels. The human HAS3 gene maps to chromosome 16q22. 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HA synthase 3; Hyaluronan synthase 3; Hyaluronate synthase 3; Hyaluronic acid synthase 3
Gene Aliases: HAS3
UniProt ID: (Human) O00219, (Mouse) O08650
Entrez Gene ID: (Human) 3038, (Mouse) 15118, (Rat) 266805
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