Invitrogen

HDAC1 Polyclonal Antibody

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HDAC1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of HDAC1 in A549 cells using a HDAC1 Polyclonal antibody (Product # PA5-120036). Blue: DAPI for nuclear staining.

HDAC1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of HDAC1 in A549 cells using a HDAC1 Polyclonal antibody (Product # PA5-120036). Blue: DAPI for nuclear staining.

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HDAC1 in human colon using a Polyclonal antibody (Product # PA5-120036) at a dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HDAC1 in human colon using a Polyclonal antibody (Product # PA5-120036) at a dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

HDAC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HDAC1 in human colon using a Polyclonal antibody (Product # PA5-120036) at a dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

HDAC1 Antibody in Western Blot (WB) — Western Blot analysis of HDAC1 in normal (control) and HDAC1 knockout (KO) 293T cells using a HDAC1 Polyclonal antibody (Product # PA5-120036) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 60s.

HDAC1 Antibody in Western Blot (WB) — Western Blot analysis of HDAC1 in normal (control) and HDAC1 knockout (KO) 293T cells using a HDAC1 Polyclonal antibody (Product # PA5-120036) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 60s.

HDAC1 Antibody in Western Blot (WB) — Western blot analysis of HDAC1 in HT-29 cells using a HDAC1 Polyclonal antibody (Product # PA5-120036) at a dilution of 1:1000.

Product Details

PA5-120036

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500 - 1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100 - 1:200

-

Immunocytochemistry (ICC/IF)

1:50 - 1:100

-

Immunoprecipitation (IP)

1:50 - 1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).

Conjugate

Unconjugated

Form

Liquid

Concentration

0.78 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2913608

Product Specific Information

Positive test controls include: 293T, HeLa, HepG2, MCF7, Mouse thymus, Mouse testis, Rat thymus, Rat testis . The target is usually found in the following locations: Nucleus.

Target Information

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686H12203; HD1; Histone deacetylase 1; Protein deacetylase HDAC1; Protein decrotonylase HDAC1; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2

Gene Aliases: GON-10; HD1; HDAC1; Hdac1-ps; MommeD5; RPD3; RPD3L1

UniProt ID: (Human) Q13547, (Mouse) O09106, (Rat) Q4QQW4

Entrez Gene ID: (Human) 3065, (Mouse) 433759, (Rat) 297893

negative regulation of transcription from RNA polymerase II promoter response to amphetamine transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein deacetylation endoderm development circadian rhythm positive regulation of cell proliferation negative regulation of cell proliferation epidermal cell differentiation negative regulation of gene expression positive regulation of receptor biosynthetic process chromatin modification histone deacetylation hippocampus development neuron differentiation positive regulation of interleukin-1 production positive regulation of tumor necrosis factor production circadian regulation of gene expression cellular response to oxidative stress odontogenesis of dentin-containing tooth positive regulation of tyrosine phosphorylation of Stat3 protein embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neuron apoptotic process negative regulation by host of viral transcription negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion rhythmic process positive regulation of oligodendrocyte differentiation negative regulation of androgen receptor signaling pathway hair follicle placode formation eyelid development in camera-type eye fungiform papilla formation histone H3 deacetylation histone H4 deacetylation negative regulation of canonical Wnt signaling pathway positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of type B pancreatic cell apoptotic process negative regulation of peptidyl-lysine acetylation negative regulation of intrinsic apoptotic signaling pathway chromatin remodeling methylation-dependent chromatin silencing blood coagulation negative regulation of myotube differentiation viral process regulation of signal transduction by p53 class mediator beta-catenin-TCF complex assembly response to caffeine response to lipopolysaccharide response to drug response to hyperoxia cellular response to tumor necrosis factor

Process(es)

transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding RNA polymerase II repressing transcription factor binding RNA polymerase II transcription corepressor activity transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding deacetylase activity enzyme binding nucleosomal DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity activating transcription factor binding histone deacetylase binding transcription regulatory region DNA binding protein N-terminus binding NF-kappaB binding repressing transcription factor binding DNA binding chromatin binding transcription corepressor activity hydrolase activity histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) protein complex binding histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)