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FIGURE: 1 / 3
HDAC4 Antibody (711390) in ICC/IF
Immunofluorescence analysis was performed on fixed and permeabilized HeLa cells for detection of endogenous HDAC4 using Anti-HDAC4 Recombinant Rabbit Superclonal™ Antibody (Product # 711390, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HDAC4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Produc... View More
Product Details
711390
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
14HCLC
Immunogen
Peptides corresponding to Human HDAC4 (aa 568-585, 147-163)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Pig and Bovine.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Histone deacetylase 4 (HDAC4) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is also involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. It is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Mutations to the HDAC4 gene have been found in Brachydactyly-mental retardation syndrome (BDMR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp779K1053; HD4; HD7; HD7b; HD9; HDAC; HDAC4; HDAC7; HDAC7B; HDAC9; HDAC9B; HDAC9FL; HDRP; Histone deacetylase 4; histone deacetylase A; KIAA0744; MITR; unnamed protein product
Gene Aliases: AHO3; BDMR; HA6116; HD4; HDAC-4; HDAC-A; HDAC4; HDACA; KIAA0288; NEDCHF; NEDCHID
UniProt ID: (Human) P56524
Entrez Gene ID: (Human) 9759
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
histone deacetylase activity
protein binding
zinc ion binding
hydrolase activity
SUMO transferase activity
potassium ion binding
protein deacetylase activity
histone binding
identical protein binding
histone deacetylase binding
metal ion binding
binding, bridging
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
histone deacetylase activity, hydrolytic mechanism
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
chromatin remodeling
protein deacetylation
inflammatory response
nervous system development
positive regulation of cell proliferation
negative regulation of macromolecule biosynthetic process
negative regulation of myotube differentiation
negative regulation of transcription by competitive promoter binding
response to denervation involved in regulation of muscle adaptation
cardiac muscle hypertrophy in response to stress
protein sumoylation
B cell differentiation
positive regulation of protein sumoylation
regulation of gene expression, epigenetic
B cell activation
negative regulation of gene expression, epigenetic
negative regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
type I interferon signaling pathway
response to interleukin-1
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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