Invitrogen

HERC5 Polyclonal Antibody

Product Image Gallery

HERC5 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of HERC5 in A431 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HERC5 polyclonal antibody (Product # PA5-114382) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-rabbit IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

HERC5 Antibody in Western Blot (WB) — Western blot analysis of HERC5 in the following samples: Lane 1: human HEK293 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in HERC5 polyclonal antibody (Product # PA5-114382) at a dilution of 0.5 µg/mL (overnight, 4°C), followed by goat anti-rabbit IgG-HRP and chemiluminescence (ECL) with a dilution of 1:10,000 (1.5 hours, room temperature).

Product Details

PA5-114382

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunocytochemistry (ICC/IF)	2 µg/mL	-
ELISA (ELISA)	0.1-0.5 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E.coli-derived human HERC5 recombinant protein (Position: F793-G1024). View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	0.05mg sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2884838

Product Specific Information

Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

HERC5 (E3 ISG15 - protein ligase HERC5) is a major E3 ligase for ISG15 conjugation. It acts a positive regulator of innate antiviral response in cells induced by interferon. HERC5 functions as a part of the ISGylation machinery that recognizes target protein in a broad and relatively non-specific manner. It catalyzes ISGylation of IRF3 - which results in sustained activation, it attenuates IRF3-PIN1 interaction - which antagonizes IFR3 ubiquitination and degradation, and boosts the antiviral repsonse. HERC5 is physically assocated with polyribosomes and broadly modifies newly synthesized proteins in a cotranslational manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Cyclin-E-binding protein 1; E3 ISG15--protein ligase HERC5; HECT domain and RCC1-like domain-containing protein 5; hect domain and RLD 5; probable E3 ubiquitin-protein ligase HERC5

Gene Aliases: CEB1; CEBP1; HERC5

UniProt ID: (Human) Q9UII4

Entrez Gene ID: (Human) 51191

Molecular Function: ubiquitin-protein ligase