Proteintech

HES5 Polyclonal Antibody

View all (10) HES5 antibodies

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HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 22666-1-AP ( HES5 Antibody) at dilution of 1:50 (under 40x lens).

HES5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 22666-1-AP (HES5 Antibody) at dilution of 1:50 (under 10x lens).

HES5 Antibody in Western Blot (WB) — Rat brain tissue were subjected to SDS PAGE followed by western blot with 22666-1-AP ( HES5 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

Product Details

22666-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HES5 Fusion Protein Ag18270 (1-172 aa encoded by BC087840)
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MRRDRINSS IEQLKLLLEQ EFARHQPNSK LEKADILEMA VSYLKHSKGE RARAPRAPSS HRAPAPPRPA ARSPPPRLPA AFVAAAGPKS LHQDYSEGYS WCLQEAVQFL TLHAASDTQM KLLYHFQRPP AAPAAPAKEP KAPGAAPPPA LSAKATAAAA AAHQPACGLW RPW (1-172 aa encoded by BC087840)

Target Information

Hairy and Enhancer-of-split homologues 5 (HES5) is a helix-loop-helix transcriptional factor that negatively regulates cell differentiation during embryogenesis. It contains one basic helix-loop-helix (bHLH) domain and one Orange domain. HES5 is reported to be a down stream effector of the Notch Signaling Pathway and is expressed in advanced ovarian serous adenocarcinomas as well as in cervical squamous carcinoma. Reports also suggest that HES5 is abundantly expressed in osteoarthritis along with Notch1 and Jagged1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Notch inhibitor can attenuate apparent diffusion coefficient and improve neurological function through downregulating NOX2-ROS in severe traumatic brain injury.

Drug design, development and therapy

Zhang HM,Chen W,Liu RN,Zhao Y

Tue Mar 26 00:00:00 EDT 2019

Notch signaling inhibitor DAPT provides protection against acute craniocerebral injury.

PloS one

Zhang HM,Liu P,Jiang C,Jin XQ,Liu RN,Li SQ,Zhao Y

Tue May 22 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: bHLHb38; Class B basic helix-loop-helix protein 38; Hairy and enhancer of split 5; RP3-395M20.5; Transcription factor HES-5

Gene Aliases: BHLHB38; Hes-5; HES5

UniProt ID: (Human) Q5TA89, (Rat) Q03062, (Mouse) P70120

Entrez Gene ID: (Human) 388585, (Rat) 79225, (Mouse) 15208

Molecular Function: basic helix-loop-helix transcription factor

negative regulation of transcription from RNA polymerase II promoter chondrocyte differentiation transcription, DNA-templated protein complex assembly cell adhesion Notch signaling pathway smoothened signaling pathway brain development positive regulation of cell proliferation oligodendrocyte development telencephalon development glial cell fate commitment forebrain radial glial cell differentiation neural tube development central nervous system myelination positive regulation of BMP signaling pathway regulation of myelination auditory receptor cell differentiation positive regulation of tyrosine phosphorylation of Stat3 protein auditory receptor cell fate determination camera-type eye development regulation of cell differentiation regulation of auditory receptor cell differentiation negative regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade cell maturation positive regulation of smooth muscle cell proliferation astrocyte differentiation negative regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation regulation of epithelial cell proliferation regulation of neurogenesis cartilage development inner ear receptor stereocilium organization comma-shaped body morphogenesis S-shaped body morphogenesis specification of loop of Henle identity metanephric nephron tubule morphogenesis establishment of epithelial cell polarity neuronal stem cell population maintenance negative regulation of stem cell differentiation negative regulation of pro-B cell differentiation negative regulation of forebrain neuron differentiation negative regulation of inner ear receptor cell differentiation transcription from RNA polymerase II promoter neuron differentiation negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development nervous system development cell differentiation

Process(es)

transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding double-stranded DNA binding transcription factor binding protein dimerization activity DNA binding protein binding