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HIF1AN Antibody (PA5-34852) in ICC/IF
HIF1AN Polyclonal Antibody detects HIF1AN protein at cytoplasm by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1AN protein stained by HIF1AN Polyclonal Antibody (Product # PA5-34852) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-34852
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 78 and 349 of HIF1AN (Uniprot ID#Q9NWT6)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2552204
Product Specific Information
Recommended positive controls: HCT116, MCF-7.^M
^M
Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (90%), Bovine (99%).^M
^M
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp762F1811; Factor inhibiting HIF-1; factor inhibiting HIF1; FIH 1; FIH-1; FLJ20615; FLJ22027; hypoxia inducible factor 1, alpha subunit inhibitor; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase
Gene Aliases: FIH1; HIF1AN
UniProt ID: (Human) Q9NWT6
Entrez Gene ID: (Human) 55662
Notch binding
iron ion binding
protein binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
oxygen sensor activity
carboxylic acid binding
peptidyl-histidine dioxygenase activity
peptidyl-asparagine 3-dioxygenase activity
protein homodimerization activity
cofactor binding
NF-kappaB binding
ankyrin repeat binding
transcription, DNA-templated
peptidyl-histidine hydroxylation
peptidyl-aspartic acid hydroxylation
peptidyl-asparagine hydroxylation
positive regulation of myoblast differentiation
negative regulation of Notch signaling pathway
oxidation-reduction process
regulation of transcription from RNA polymerase II promoter in response to hypoxia
negative regulation of transcription from RNA polymerase II promoter in response to hypoxia
positive regulation of vasculogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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