MA3-413 detects heat shock cognate protein 70 kDa (HSC70)-interacting protein (Hip) from human, mouse, chicken and rabbit samples.
MA3-413 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a 48 kDa protein representing Hip from rabbit reticulocyte lysate.
The MA3-413 antigen is recombinant human Hip protein.
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP90, HSP70), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 and FKBP54 and the cyclosporin binding protein, CyP-40) and three other proteins termed p23, p60 and heat shock cognate protein 70 kDa (HSC70)-interacting protein (Hip), also known as p48. Hip, along with HSP70 and p60, combine with steroid receptors as transient intermediate complex members.
In vitro studies have suggested that Hip transiently interacts with progesterone receptor in a cell free system prior to hormone binding competency and dissociates from the receptor complex with the binding of an HSP90:Immunophilin:p23 subcomplex which then results in a hormone-binding-competent receptor complex. While neither its exact function nor mechanism of action have been identified, Hip appears to be an important factor in steroid receptor function. Hip also appears to regulate the function of HSC70 in an ATP hydrolysis and HSP40 dependent fashion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AAG2; Aging-associated protein 2; FAM10A1; heat shock 70kD protein binding protein; HIP; Hsc70-interacting protein; Hsp70 interacting protein; Hsp70-interacting protein; Progesterone receptor-associated p48 protein; Protein FAM10A1; Protein ST13 homolog; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; SNC6; Suppression of tumorigenicity 13 protein; testis secretory sperm-binding protein Li 233m
Gene Aliases: 1110007I03Rik; 3110002K08Rik; AAG2; AW555194; FAM10A1; FAM10A4; HIP; HOP; HSPABP; HSPABP1; P48; PRO0786; RCJMB04_6h13; SNC6; ST13
Molecular Function: chaperone