Invitrogen

HIPPI Monoclonal Antibody (OTI4F6)

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HIPPI Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of IFT57 in COS7 cells. Cells were transfected with a plasmid overexpressing IFT57 and probed with a IFT57 monoclonal antibody (Product # MA5-25044).

HIPPI Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a IFT57 monoclonal antibody (Product # MA5-25044) at a dilution of 1:50.

HIPPI Antibody in Western Blot (WB) — Western blot analysis of IFT57 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with IFT57 (Product # MA5-25044) monoclonal antibody.

Product Details

MA5-25044

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI4F6
Immunogen	Full length human recombinant protein of IFT57 produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724451

Target Information

HIPPI is required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Dermal papilla-derived protein 8; estrogen-related receptor beta like 1; Estrogen-related receptor beta-like protein 1; FLJ10147; HIP1 protein interactor; HIP1-interacting protein; huntingtin interacting protein-1 interacting protein; intraflagellar transport 57 homolog; Intraflagellar transport protein 57 homolog; MHS4R2

Gene Aliases: DERP8; ESRRBL1; HIPPI; IFT57; MHS4R2

UniProt ID: (Human) Q9NWB7

Entrez Gene ID: (Human) 55081

Molecular Function: structural protein