Invitrogen

HIPPI Monoclonal Antibody (OTI4F6)

Product Image Gallery

HIPPI Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of IFT57 in COS7 cells. Cells were transfected with a plasmid overexpressing IFT57 and probed with a IFT57 monoclonal antibody (Product # MA5-25044).

HIPPI Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a IFT57 monoclonal antibody (Product # MA5-25044) at a dilution of 1:50.

HIPPI Antibody in Western Blot (WB) — Western blot analysis of IFT57 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with IFT57 (Product # MA5-25044) monoclonal antibody.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI4F6

Immunogen

Full length human recombinant protein of IFT57 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724451

Target Information

Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Dermal papilla-derived protein 8; ESRRBL1; estrogen-related receptor beta like 1; Estrogen-related receptor beta-like protein 1; HIP1 protein interactor; HIP1-interacting protein; HIPPI; huntingtin interacting protein-1 interacting protein; intraflagellar transport 57 homolog; intraflagellar transport protein 57; Intraflagellar transport protein 57 homolog; MHS4R2

Gene Aliases: DERP8; ESRRBL1; HIPPI; IFT57; MHS4R2

UniProt ID: (Human) Q9NWB7

Entrez Gene ID: (Human) 55081

Molecular Function: cytoskeletal protein enzyme modulator