|Tested species reactivity||Virus|
|Host / Isotype||Guinea pig / IgG|
|Immunogen||Recombinant HIV-1 Reverse Transcriptase protein|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:2500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4950 detects the HIV-1 Reverse Transcriptase protein in HIV samples.
PA1-4950 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects a ~66 kDa protein and a ~51 kDa protein, corresponding respectively to the two subunits of HIV-1 reverse transcriptase, in HIV samples.
PA1-4950 immunogen corresponds to the HIV-1 Reverse Transcriptase which was over-expressed in E. coli as a recombinant protein.
Recombinant HIV-1 Reverse Transcriptase protein (Cat. # RP-4912 or RP-4913) can be purchased for control experiments in Western blot assays.
HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1(AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.