Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
HLTF Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 3
HLTF Antibody (PA5-79371) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of HLTF in A549 cell using HLTF Polyclonal Antibody (Product # PA5-79371). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL and mouse anti-Tubulin Alpha antibody overnight at 4°C. Cy3 conjugated goat anti-rabbit IgG and DyLight 488 conjugated goat anti-mouse IgG were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visual... View More
Product Details
PA5-79371
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human HLTF recombinant protein (Position: S911-L1009).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human PANC-1 whole cell, human A549 whole cell, rat heart tissue, rat kidney tissue, mouse heart tissue, mouse kidney tissue. ICC/IF: A549 cell. Flow: CACO-2 cell.
Target Information
This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DNA-binding protein/plasminogen activator inhibitor 1 regulator; DNA-binding protein/plasminogen activator inhibitor-1 regulator; Helicase-like transcription factor; HIP116; mem; P113; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; SNF2-like 3; Sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; TNF-response element-binding protein
Gene Aliases: AF010600; BC057116; HIP116; HIP116A; HLTF; HLTF1; P113; RNF80; SMARCA3; SNF2L3; ZBU1
UniProt ID: (Human) Q14527, (Mouse) Q6PCN7
Entrez Gene ID: (Human) 6596, (Mouse) 20585, (Rat) 295568
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
helicase activity
protein binding
ATP binding
zinc ion binding
ligase activity
ATPase activity
ubiquitin protein ligase binding
poly(A) RNA binding
ubiquitin protein ligase activity
nucleotide binding
nucleic acid binding
catalytic activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
protein polyubiquitination
postreplication repair
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
covalent chromatin modification
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
metabolic process
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support