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Invitrogen
HMGA1 Polyclonal Antibody
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FIGURE: 1 / 6
HMGA1 Antibody (PA5-78007) in ICC/IF
Immunofluorescence analysis of HMGA1 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HMGA1 Polyclonal Antibody (Product # PA5-78007) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Product Details
PA5-78007
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human HMGA1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.87 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Mouse (100%), Rat (100%), Dog (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:5010; High mobility group AT-hook protein 1; High mobility group protein A1; High mobility group protein HMG-I/HMG-Y; High mobility group protein R; high-mobility group (nonhistone chromosomal) protein isoforms I and Y; HMG-I(Y); MGC12816; MGC4242; MGC4854; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; OTTHUMP00000016222; OTTHUMP00000016223; OTTHUMP00000016224; OTTHUMP00000039618
Gene Aliases: HMG-R; HMGA1; HMGA1A; HMGIY
UniProt ID: (Human) P17096
Entrez Gene ID: (Human) 3159
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
DNA binding
AT DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
enzyme binding
ligand-dependent nuclear receptor transcription coactivator activity
retinoic acid receptor binding
peroxisome proliferator activated receptor binding
retinoid X receptor binding
endodeoxyribonuclease
DNA unwinding involved in DNA replication
nucleosome disassembly
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein complex assembly
negative regulation of cell proliferation
response to virus
negative regulation of chromatin silencing
senescence-associated heterochromatin focus assembly
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
nuclear transport
establishment of integrated proviral latency
oncogene-induced cell senescence
positive regulation of cellular senescence
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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