Hamburger Menu Button
Thermo Fisher Scientific Logo
登入
還沒有帳戶嗎 ? 立即註冊
  • 產品
    • Antibodies
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Cell Culture Media
    • Chemicals
    • Western Blot Products
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Greener Products
    • 查看所有產品類別
  • 應用
    • 生物製程
    • 細胞培養和轉染
    • 食品和飲料
    • 實驗室解決方案
    • 製藥和生物製藥
    • 即時定量PCR
    • 半導體分析
    • 臨床和診斷
    • 數位解決方案
    • 分子光譜、同位素與元素分析
    • 查看所有應用與技術
  • 服務
    • 360° CDMO 和 CRO 服務
    • CDMO 服务
    • 临床试验 CRO 服务
    • 客製化服務
    • 金融與租賃服務
    • 儀器服務
    • 實驗室資訊學
    • 授權與商業供應
    • 培訓服務
    • 查看所有服務
  • 支援
    • 線上訂購協助
    • 數位解決方案
    • 產品支持
    • 技術相關資訊
    • 培訓與教育
    • 查看所有協助與支援
  • 熱門商品
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • 我們的服務對象
    • 生物技術
    • 生物製藥
    • CDMO
    • 實驗室診斷
    • 工業與應用科學
  • 特價商品
  • 聯絡我們
  • 快速訂購
  • 訂單狀態與貨物追蹤
  • 文件和證書
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • 訂單查詢
          • 快速訂購
          • 登入
            登入
            還沒有帳戶嗎 ? 立即註冊
            • 帳戶
            • 訂單
            • Connect: Lab, Data, Apps
            • 客製化產品與項目
            • 儀器管理
          • Primary Antibodies ›
          • HMGB1 Antibodies

          Invitrogen

          HMGB1 Polyclonal Antibody

          Advanced Verification
          1 Reference
          View all (59) HMGB1 antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Tech Support

          Cite HMGB1 Polyclonal Antibody

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (10)
          • Advanced Verification (1)
          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          HMGB1 Antibody (PA5-29604) in ICC/IF

          HMGB1 Polyclonal Antibody detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 Polyclonal Antibody (Product # PA5-29604) diluted at 1:1,000. Blue: Hoechst 33342 staining. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          HMGB1 Antibody in Immunocytochemistry (ICC/IF)
          HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HMGB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody in Western Blot (WB)
          HMGB1 Antibody

          Product Details

          PA5-29604

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat, Zebrafish

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.53 mg/mL

          Amount

          53 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 10% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2547080

          Product Specific Information

          Recommended positive controls: 293T, HepG2, mouse brain, PC-12, Rat2, rat brain.

          Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (92%), Dog (100%), Pig (99%), Rabbit (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%).

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          High-mobility group box 1 protein, previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Amphoterin; Amphoterin antibody; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG-1; HMG3 antibody; hmgb 1; hmgb-1; HMGB1; RP11-550P23.1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product

          View more View less

          Gene Aliases: Ac2-008; amphoterin; DEF; HMG-1; HMG1; HMG3; HMGB1; p30; SBP-1

          View more View less

          UniProt ID: (Human) P09429, (Rat) P63159, (Mouse) P63158

          View more View less

          Entrez Gene ID: (Human) 3146, (Rat) 25459, (Mouse) 15289

          View more View less

          Function(s)
          four-way junction DNA binding bubble DNA binding transcription regulatory region sequence-specific DNA binding lipopolysaccharide binding phosphatidylserine binding DNA binding damaged DNA binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity transcription corepressor activity RNA binding double-stranded RNA binding single-stranded RNA binding cytokine activity integrin binding protein binding lipid binding DNA binding, bending calcium-dependent protein kinase regulator activity lyase activity C-X-C chemokine binding protein kinase activator activity chemoattractant activity receptor agonist activity RAGE receptor binding RNA polymerase II sequence-specific DNA binding transcription factor binding DNA polymerase binding supercoiled DNA binding DNA-binding transcription factor binding open form four-way junction DNA binding crossed form four-way junction DNA binding bent DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding 5S rRNA binding transcription factor binding heparin binding peptide binding poly(A) RNA binding protein dimerization activity glycolipid binding repressing transcription factor binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter eye development myeloid dendritic cell activation endothelial cell proliferation positive regulation of endothelial cell proliferation activation of innate immune response adaptive immune response plasmacytoid dendritic cell activation macrophage activation involved in immune response myeloid progenitor cell differentiation immune system process dendritic cell chemotaxis inflammatory response to antigenic stimulus regulation of tolerance induction regulation of T cell mediated immune response to tumor cell glycogen catabolic process DNA metabolic process DNA topological change DNA repair base-excision repair double-strand break repair double-strand break repair via nonhomologous end joining DNA recombination chromatin organization chromatin remodeling transcription from RNA polymerase II promoter autophagy chemotaxis inflammatory response immune response cellular response to DNA damage stimulus signal transduction positive regulation of cytosolic calcium ion concentration positive regulation of autophagy negative regulation of endothelial cell migration negative regulation of RNA polymerase II transcriptional preinitiation complex assembly myeloid cell differentiation lung development neuron projection development heterochromatin assembly regulation of restriction endodeoxyribonuclease activity DNA geometric change positive regulation of mismatch repair negative regulation of interferon-gamma production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production V(D)J recombination positive regulation of toll-like receptor 2 signaling pathway positive regulation of toll-like receptor 4 signaling pathway positive regulation of toll-like receptor 9 signaling pathway T-helper 1 cell activation endothelial cell chemotaxis positive regulation of activated T cell proliferation positive regulation of apoptotic process apoptotic cell clearance negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of DNA binding positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration T-helper 1 cell differentiation innate immune response positive regulation of innate immune response positive regulation of cell differentiation positive regulation of myeloid cell differentiation positive regulation of glycogen catabolic process positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of viral entry into host cell cell development regulation of viral process positive chemotaxis regulation of DNA metabolic process response to glucocorticoid positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide positive regulation of monocyte chemotactic protein-1 production positive regulation of monocyte chemotaxis positive regulation of wound healing neutrophil clearance cellular response to interleukin-7 positive regulation of NIK/NF-kappaB signaling positive regulation of sprouting angiogenesis regulation of hemopoiesis positive regulation of myeloid progenitor cell differentiation positive regulation of vascular endothelial cell proliferation positive regulation of chemokine (C-X-C motif) ligand 2 production negative regulation of apoptotic cell clearance regulation of nucleotide-excision repair positive regulation of dendritic cell differentiation cell morphogenesis positive regulation of protein phosphorylation positive regulation of mesenchymal cell proliferation base-excision repair, DNA ligation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter nervous system development circadian rhythm negative regulation of DNA replication positive regulation of cell proliferation response to heat response to glucose positive regulation of cell death positive regulation of neuron projection development positive regulation of smooth muscle cell migration positive regulation of cell migration actin cytoskeleton reorganization response to lipopolysaccharide response to insulin positive regulation of myeloid cell apoptotic process response to interferon-gamma response to drug positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of myoblast differentiation positive regulation of protein kinase activity positive regulation of mitotic cell cycle regulation of inflammatory response male-specific defense response to bacterium induction of positive chemotaxis myoblast proliferation cellular response to interleukin-1
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          產品訂購 Plus Icon Minus Icon
          • 訂單狀態
          • 訂購協助
          • 快速訂購
          • 現貨供應中心
          • 電子採購
          支持 Plus Icon Minus Icon
          • 協助與支持
          • 聯絡我們
          • 技術支持中心
          • 搜尋文件和證書
          • 回報網站問題
          資源 Plus Icon Minus Icon
          • 學習中心
          • 促銷活動
          • 活動和網路研討會
          • 社群媒體
          關於 Thermo Fisher Plus Icon Minus Icon
          • 關於我們 關於我們
          • 人才招募 人才招募
          • 投資者資訊 投資者資訊
          • 新聞 新聞
          • 企業社會責任 企業社會責任
          • 商標
          • 政策和聲明
          品牌 Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Policy
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          Taiwan flag icon
          Taiwan

          Your items have has been added!


          Host server : magellan-search-blue-744bc48644-454p7:80/100.66.72.101:80.
          git-commit: 747bde55a712f6f97bf7760408d445eefba4e16f
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.48.0-Offline