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HMGCS1 Polyclonal Antibody

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HMGCS1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using HMGCS1 antibody 17643-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

HMGCS1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 17643-1-AP (HMGCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HMGCS1 Antibody in Immunoprecipitation (IP) — IP result of anti-HMGCS1 (IP:17643-1-AP, 3ug; Detection:17643-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

Product Details

17643-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 8 publications 8 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HMGCS1 Fusion Protein Ag11825 (171-520 aa encoded by BC083514) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: TGGVGAVAL LIGPNAPLIF ERGLRGTHMQ HAYDFYKPDM LSEYPIVDGK LSIQCYLSAL DRCYSVYCKK IHAQWQKEGN DKDFTLNDFG FMIFHSPYCK LVQKSLARML LNDFLNDQNR DKNSIYSGLE AFGDVKLEDT YFDRDVEKAF MKASSELFSQ KTKASLLVSN QNGNMYTSSV YGSLASVLAQ YSPQQLAGKR IGVFSYGSGL AATLYSLKVT QDATPGSALD KITASLCDLK SRLDSRTGVA PDVFAENMKL REDTYHLVNY IPQGSIDSLF EGTWYLVRVD EKHRRTYARR PTPNDDTLDE GVGLVHSNIA TEHIPSPAKK VPRLPATAAE PEAAVISNGE H (171-520 aa encoded by BC083514)

Target Information

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain.

Neuroscience

Raihan O,Brishti A,Molla MR,Li W,Zhang Q,Xu P,Khan MI,Zhang J,Liu Q

Mon Oct 15 00:00:00 EDT 2018

Potent effects of dioscin against obesity in mice.

Scientific reports

Liu M,Xu L,Yin L,Qi Y,Xu Y,Han X,Zhao Y,Sun H,Yao J,Lin Y,Liu K,Peng J

Thu Jan 22 00:00:00 EST 2015

Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.

Food & function

Dong D,Qi Y,Xu L,Yin L,Xu Y,Han X,Zhao Y,Peng J

Mon Dec 01 00:00:00 EST 2014

Protective effects of dioscin against alcohol-induced liver injury.

Archives of toxicology

Xu T,Zheng L,Xu L,Yin L,Qi Y,Xu Y,Han X,Peng J

Sat Mar 01 00:00:00 EST 2014

Protective effects of dioscin against alcohol-induced liver injury.

Archives of toxicology

Xu T,Zheng L,Xu L,Yin L,Qi Y,Xu Y,Han X,Peng J

Sat Mar 01 00:00:00 EST 2014

The endoplasmic reticulum coat protein II transport machinery coordinates cellular lipid secretion and cholesterol biosynthesis.

The Journal of biological chemistry

Fryer LG,Jones B,Duncan EJ,Hutchison CE,Ozkan T,Williams PA,Alder O,Nieuwdorp M,Townley AK,Mensenkamp AR,Stephens DJ,Dallinga-Thie GM,Shoulders CC

Fri Feb 14 00:00:00 EST 2014

Identification of proteins that mediate the pro-viral functions of the interferon stimulated gene 15 in hepatitis C virus replication.

Antiviral research

Real CI,Megger DA,Sitek B,Jahn-Hofmann K,Ickenstein LM,John MJ,Walker A,Timm J,Kuhlmann K,Eisenacher M,Meyer HE,Gerken G,Broering R,Schlaak JF

Sun Dec 01 00:00:00 EST 2013

Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats.

Food chemistry

Zhang S,Zheng L,Dong D,Xu L,Yin L,Qi Y,Han X,Lin Y,Liu K,Peng J

Sun Dec 01 00:00:00 EST 2013

Quantitative proteomics reveals that enzymes of the ketogenic pathway are associated with prostate cancer progression.

Molecular & cellular proteomics : MCP

Saraon P,Cretu D,Musrap N,Karagiannis GS,Batruch I,Drabovich AP,van der Kwast T,Mizokami A,Morrissey C,Jarvi K,Diamandis EP

Sat Jun 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); HMG-CoA synthase; hydroxymethylglutaryl-CoA synthase 1; Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene Aliases: B130032C06Rik; HMGCS; HMGCS1

UniProt ID: (Human) Q01581, (Rat) P17425, (Mouse) Q8JZK9

Entrez Gene ID: (Human) 3157, (Rat) 29637, (Mouse) 208715

Function(s)

liver development cholesterol biosynthetic process brain development isoprenoid biosynthetic process male gonad development response to low light intensity stimulus response to hormone response to organic substance response to organonitrogen compound response to organic cyclic compound response to purine-containing compound response to vitamin E response to gonadotropin response to drug response to tellurium ion response to lipoprotein particle response to cholesterol cellular response to follicle-stimulating hormone stimulus cellular response to cholesterol cellular response to organic cyclic compound lipid metabolic process steroid biosynthetic process metabolic process steroid metabolic process cholesterol metabolic process sterol biosynthetic process

Process(es)

hydroxymethylglutaryl-CoA synthase activity drug binding isomerase activity protein homodimerization activity organic acid binding catalytic activity transferase activity

HMGCS1 Polyclonal Antibody

Product Details

17643-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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