Invitrogen

HMOX1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (25) HO-1 antibodies

Questions & Answers

Questions & Answers

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HMOX1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of HMOX1 in human spleen and salivary gland tissues using a HMOX1 polyclonal antibody (Product # PA5-81883). Corresponding RNA-seq data are presented for the same tissues.

HMOX1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of HMOX1 in human salivary gland using a HMOX1 polyclonal antibody (Product # PA5-81883). The analysis shows low expression as expected.

HMOX1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of HMOX1 in human spleen using a HMOX1 polyclonal antibody (Product # PA5-81883). The analysis shows high expression.

HMOX1 Antibody in Western Blot (WB) — Western blot analysis of HMOX1 by a HMOX1 polyclonal antibody (Product # PA5-81883). Analysis in human cell lines A-549 and MCF-7.

HMOX1 Antibody in Western Blot (WB) — Western blot analysis of HMOX1 by a HMOX1 polyclonal antibody (Product # PA5-81883). Analysis in human cell line HepG2.

HMOX1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of HMOX1 demonstrates antibody specificity. Immunohistochemical analysis of HMOX1 using anti-HMOX1 Polyclonal Antibody (Product #PA5-81883), shows significant staining of HMOX1 in spleen and shows minimal or weak staining in salivary gland tissues. The relative expression levels of HMOX1 within each tissue is shown using RNA-Seq. {RE}

HMOX1 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of HMOX1 was observed specifically in A-549 cells and in MCF-7 cells using anti-HMOX1 Polyclonal Antibody (Product #PA5-81883) in western blot. The relative expression levels of HMOX1 within each cell line is shown using RNA-Seq. {RE}

Product Details

PA5-81883

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human HMOX1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2789044

Product Specific Information

Immunogen sequence: QDLSEALKEA TKEVHTQAEN AEFMRNFQKG QVTRDGFKLV MASLYHIYVA LEEEIERNKE SPVFAPVYFP EELHRKAALE QDLAFWYGPR WQEVIPYTPA MQRYVKRLHE VGRTEPELLV AHAYTRYLGD LSGGQVLKKI AQKALDLPSS GEGLAFFTFP NIASATKFKQ LYRSRMNSLE MTPAVRQRVI EEAKTAF

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; Heme oxygenase 1; HO-1

Gene Aliases: bK286B10; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

UniProt ID: (Human) P09601

Entrez Gene ID: (Human) 3162

Molecular Function: oxygenase

angiogenesis endothelial cell proliferation wound healing involved in inflammatory response negative regulation of leukocyte migration heme oxidation cellular iron ion homeostasis response to oxidative stress small GTPase mediated signal transduction excretion regulation of blood pressure cell death intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process smooth muscle hyperplasia cellular response to nutrient negative regulation of mast cell cytokine production erythrocyte homeostasis low-density lipoprotein particle clearance regulation of transcription from RNA polymerase II promoter in response to iron cellular response to heat response to nicotine intracellular signal transduction heme catabolic process response to hydrogen peroxide positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen positive regulation of chemokine biosynthetic process regulation of angiogenesis positive regulation of angiogenesis positive regulation of vasodilation positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion cellular response to hypoxia negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Process(es)

heme oxygenase (decyclizing) activity phospholipase D activity signal transducer activity protein binding enzyme binding heme binding protein homodimerization activity metal ion binding