Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:500 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide of N-terminal human Heme Oxygenase 1 (HO-1) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
Contains |
0.05% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Positive Control: Mouse spleen tissue lysate, 293T, HepG2, human spleen tissue, human placenta tissue, human small intestine tissue, mouse small intestine tissue.
Subcellular Location: Endoplasmic reticulum.
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; Heme oxygenase 1; hemoxygenase; HO-1; P32 protein
Gene Aliases: bK286B10; D8Wsu38e; Hemox; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32
UniProt ID: (Human) P09601, (Mouse) P14901
Entrez Gene ID: (Human) 3162, (Mouse) 15368
Molecular Function:
oxygenase
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