Invitrogen

HOMER1 Polyclonal Antibody

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HOMER1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HOMER1 using a polyclonal antibody (Product # PA5-23023).

HOMER1 Antibody in Western Blot (WB) — Western blot was performed using Anti-HOMER1 Polyclonal Antibody (Product # PA5-23023) and a 40kDa band corresponding to HOMER1 was observed across in Mouse Brain, Mouse Skeletal Muscle, Rat Brain, Rat Skeletal Muscle but was not observed in Mouse Kidney, Mouse Spleen, Rat Kidney and Rat Spleen which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of Mouse Brain (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Kidney (Lane 3), Mouse Spleen (Lane 4), Rat Brain (Lane 5), Rat Skeletal Muscle (Lane 6), Rat Kidney (Lane 7), Rat Spleen (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

PA5-23023

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	N-terminal partial recombinant human HOMER protein expressed in E. coli. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.1% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11154279

Product Specific Information

The target sequence has 99% sequence homology with rat, 98% to bovine, 96% to xenopus and 74% to zebrafish.

Target Information

HOMER1 is a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homer homolog 1; Homer protein homolog 1; homer, neuronal immediate early gene, 1; Homer-1; immediate early gene protein Homer1A; VASP/Ena-related gene up-regulated during seizure and LTP 1; Vesl-1

Gene Aliases: HOMER; homer-1; HOMER1; HOMER1A; HOMER1B; HOMER1C; PSD-Zip45; SYN47; Ves-1; vesl-1; Vesl1

UniProt ID: (Human) Q86YM7, (Mouse) Q9Z2Y3

Entrez Gene ID: (Human) 9456, (Mouse) 26556

Function(s)