Invitrogen

HOMER1 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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HOMER1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of HOMER1 in HeLa cells. Samples were incubated in HOMER1 polyclonal antibody (Product # PA5-23023). HOMER1 (Green). Nuclei (Blue) are counterstained using Hoechst 33258..

HOMER1 Antibody in Western Blot (WB) — Western blot was performed using Anti-HOMER1 Polyclonal Antibody (Product # PA5-23023) and a 40kDa band corresponding to HOMER1 was observed across in Mouse Brain, Mouse Skeletal Muscle, Rat Brain, Rat Skeletal Muscle but was not observed in Mouse Kidney, Mouse Spleen, Rat Kidney and Rat Spleen which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of Mouse Brain (Lane 1), Mouse Skeletal Muscle (Lane 2), Mouse Kidney (Lane 3), Mouse Spleen (Lane 4), Rat Brain (Lane 5), Rat Skeletal Muscle (Lane 6), Rat Kidney (Lane 7), Rat Spleen (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

PA5-23023

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

N-terminal partial recombinant human HOMER protein expressed in E. coli.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154279

Product Specific Information

The target sequence has 99% sequence homology with rat, 98% to bovine, 96% to xenopus and 74% to zebrafish.

Target Information

HOMER1 is a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homer homolog 1; Homer protein homolog 1; homer, neuronal immediate early gene, 1; Homer-1; immediate early gene protein Homer1A; VASP/Ena-related gene up-regulated during seizure and LTP 1; Vesl-1

Gene Aliases: HOMER; homer-1; HOMER1; HOMER1A; HOMER1B; HOMER1C; PSD-Zip45; SYN47; Ves-1; vesl-1; Vesl1

UniProt ID: (Human) Q86YM7, (Mouse) Q9Z2Y3

Entrez Gene ID: (Human) 9456, (Mouse) 26556

Function(s)

skeletal muscle contraction G-protein coupled glutamate receptor signaling pathway protein localization to synapse behavioral response to cocaine skeletal muscle fiber development chemical homeostasis within a tissue response to calcium ion positive regulation of calcium ion transport regulation of calcium ion import regulation of store-operated calcium entry regulation of cation channel activity phospholipase C-activating G-protein coupled glutamate receptor signaling pathway chemical synaptic transmission positive regulation of signal transduction

Process(es)

protein binding G-protein coupled glutamate receptor binding signaling adaptor activity ion channel binding receptor binding type 5 metabotropic glutamate receptor binding protein complex scaffold identical protein binding protein heterodimerization activity scaffold protein binding