|Tested species reactivity||Human|
|Published species reactivity||Pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 122 and 310 of Human HSD17B3|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-30063 targets HSD17B3 in IHC (P), ICC, IF and WB applications and shows reactivity with Human samples.
The PA5-30063 immunogen is recombinant fragment corresponding to a region within amino acids 122 and 310 of Human HSD17B3.
This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Altered expression of 3ß-HSD, CYP17 and 17ß-HSD in the foetal porcine gonads in response to anti-androgen flutamide exposure.
PA5-30063 was used in immunohistochemistry to test if androgen depletion alters expression of steroidogenic enzymes during late prenatal period
|Knapczyk-Stwora K,Grzesiak M,Slomczynska M||Reproduction in domestic animals = Zuchthygiene (49:725)||2014|