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Bethyl Laboratories
HSF1 Polyclonal Antibody
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FIGURE: 1 / 3
HSF1 Antibody (A303-174A) in IHC (P)
Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSF1 (Product # A303-174A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (left), (Product # A303-175A) (Lot 1) used at a dilution of 1:5,000 (0.2 µg/mL) (center) and (Product # A303-176A) (Lot 1) used at a dilution of 1:1,000 (0.2 µg/mL) (right). Detection: DAB.
Product Details
A303-174A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 225 and 275 of human Heat Shock Transcription Factor 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSTF 1
Gene Aliases: HSF1; HSTF1
UniProt ID: (Human) Q00613
Entrez Gene ID: (Human) 3297
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
embryonic placenta development
protein phosphorylation
defense response
female meiotic division
spermatogenesis
negative regulation of cell proliferation
mRNA transcription
response to lipopolysaccharide
negative regulation of tumor necrosis factor production
cellular response to heat
positive regulation of multicellular organism growth
positive regulation of transcription from RNA polymerase II promoter
embryonic process involved in female pregnancy
regulation of cellular response to heat
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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