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Agrisera
HTA9/Probable histone H2A variant 3 Polyclonal Antibody
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FIGURE: 1 / 1
HTA9/Probable histone H2A variant 3 Antibody (AS10-718) in WB
1) hta 9, hta 11 double mutant, about 1 µg protein; 2) mutant hta 9, hta 11 about 2 µg protein; 3) wild-type about 2-3 µg protein; 4) wild-type about 4-5 µg protein from Arabidopsis thaliana. Protein from Arabidopsis histone preparation (acid extracted, precipitated with acetone and resuspended in urea). Denatured with SDS-PAGE buffer at 90 C for 2 min, separated on 15% SDS-PAGE and blotted 1 h to PVDF tank... View More
Product Details
AS10-718
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HTA9.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
HTA9 encodes a non-canonical histone H2A variant of the H2A.Z class that localizes to the nucleus and is incorporated into chromatin as part of nucleosomes, thereby influencing local chromatin architecture and DNA accessibility. The gene is a compact, intron-containing locus (two exons) that produces an H2A-family protein expected to adopt the conserved histone fold required for H2A-H2B dimerization within the nucleosome core, while retaining H2A.Z-specific sequence features that distinguish it from canonical replication-coupled H2A. Biologically, HTA9 contributes to H2A.Z-dependent regulation of transcriptional programs that respond to developmental and environmental cues: genetic loss of H2A.Z function (including HTA9 in combination with other H2A.Z-encoding genes) leads to widespread gene misregulation, and has been linked to altered flowering-related regulation and temperature-responsive gene expression as well as changes in epigenetic states such as reduced transposon DNA methylation without broadly reducing genic methylation, consistent with a role for H2A.Z-containing nucleosomes in shaping plant chromatin function, genome stability, and signal-responsive gene control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT1G52740; F14G24.1; F14G24_1; H2A.Z
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