|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 587 and 826 of Human Hexokinase III|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-29304 targets Hexokinase III in IF and WB applications and shows reactivity with Human samples.
The PA5-29304 immunogen is recombinant fragment corresponding to a region within amino acids 587 and 826 of Human Hexokinase III.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effect of lentivirus-mediated shRNA inactivation of HK1, HK2, and HK3 genes in colorectal cancer and melanoma cells.
PA5-29304 was used in western blot to elucidate the role of hexokinases in colorectal cancer growth
|Kudryavtseva AV,Fedorova MS,Zhavoronkov A,Moskalev AA,Zasedatelev AS,Dmitriev AA,Sadritdinova AF,Karpova IY,Nyushko KM,Kalinin DV,Volchenko NN,Melnikova NV,Klimina KM,Sidorov DV,Popov AY,Nasedkina TV,Kaprin AD,Alekseev BY,Krasnov GS,Snezhkina AV||BMC genetics (17:null)||2016|