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FIGURE: 1 / 1
Hif3 alpha Antibody (600-401-435) in WB
Western blot using Rocklands Affinity Purified anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A (arrowhead). Approximately 10 µg of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 µM CoCl2 for 6 h at 37° C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4° C with the primary antibody diluted to 1:1,6... View More
Product Details
600-401-435
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against mouse Hif3alpha protein. A BLAST analysis was used to suggest cross reactivity with Hif3a protein from mouse (100% homology) and rat (91% homology). Reactivity against human Hif3a is not expected as only 75% homology for the immunizing sequence is noted. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.
Target Information
Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1 alpha, HIF-1 beta (also designated Arnt 1), EPAS-1 (also designated HIF-2 alpha) and HIF-3 alpha, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1 alpha is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Basic-helix-loop-helix-PAS protein MOP7; HIF-3-alpha; HIF3-alpha; HIF3-alpha-1; hypoxia inducible factor three alpha; Hypoxia-inducible factor 3-alpha; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; Neonatal and embryonic PAS protein
Gene Aliases: bHLHe17; Hif3a; Ipas; MOP7; NEPAS
UniProt ID: (Mouse) Q0VBL6
Entrez Gene ID: (Mouse) 53417
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
transcription corepressor activity
signal transducer activity
protein binding
protein dimerization activity
basic helix-loop-helix transcription factor
angiogenesis
response to hypoxia
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
apoptotic process
signal transduction
multicellular organism development
positive regulation of transcription from RNA polymerase II promoter
negative regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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