Invitrogen

Histone H1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Histone H1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Histone H1 was performed using 70% confluent log phase U-2OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone H1 Rabbit Polyclonal Antibody (Product # PA5-30055) at 5 microgram/mL concentration in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Histone H1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Histone H1 Polyclonal Antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H1 Polyclonal Antibody (Product # PA5-30055) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Histone H1 Antibody in Western Blot (WB) — Western blot analysis was performed on acid extracts (20 µg lysate) of NTERA-2 (Lane 1), U-2 OS (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5) and LNCaP (Lane 6). The blot was probed with Anti-Histone H1 (Product # PA5-30055, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 30 kDa band corresponding to Histone H1 was observed across the cell lines tested.

Product Details

PA5-30055

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:20,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 1 and 194 of Human Histone H1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547529

Product Specific Information

Recommended positive controls: HepG2, HepG2 nuclear extract, mouse liver.

Predicted reactivity: Mouse (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift so it is unlikely to encode a functional protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A peptide of the N terminus of GRK5 attenuates pressure-overload hypertrophy and heart failure.

Science signaling

Coleman RC,Eguchi A,Lieu M,Roy R,Barr EW,Ibetti J,Lucchese AM,Peluzzo AM,Gresham K,Chuprun JK,Koch WJ

PA5-30055 was used in Western Blotting to support a role for GRK5nt as an inhibitor of pathological GRK5 signaling that prevents heart failure.

Tue Mar 30 00:00:00 EDT 2021

The 20S proteasome activator PA28γ controls the compaction of chromatin.

Journal of cell science

Fesquet D,Llères D,Grimaud C,Viganò C,Méchali F,Boulon S,Coux O,Bonne-Andrea C,Baldin V

PA5-30055 was used in Western Blot to show that PA28γ controls chromatin compaction.

Mon Feb 01 00:00:00 EST 2021

Linking epigenetic dysregulation, mitochondrial impairment, and metabolic dysfunction in SBMA motor neurons.

JCI insight

Pourshafie N,Masati E,Bunker E,Nickolls AR,Thepmankorn P,Johnson K,Feng X,Ekins T,Grunseich C,Fischbeck KH

PA5-30055 was used in Western Blotting to show a molecular link between altered epigenetic regulation and mitochondrial metabolism that contributes to neurodegeneration.

Thu Jul 09 00:00:00 EDT 2020

Cx43 and AKAP95 regulate G1/S conversion by competitively binding to cyclin E1/E2 in lung cancer cells.

Thoracic cancer

Chen R,Chen Y,Yuan Y,Zou X,Sun Q,Lin H,Chen X,Liu M,Deng Z,Yao Y,Guo D,Zhang Y

PA5-30055 was used in western blotting to determine how the competitive binding of Cx43 and AKAP95 to cyclin E1/E2 affects G1/S conversion in lung cancer cells.

Mon Jun 01 00:00:00 EDT 2020

A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants.

Biochemistry

Osunsade A,Prescott NA,Hebert JM,Ray DM,Jmeian Y,Lorenz IC,David Y

PA5-30055 was used in Western blot to present a robust method to purify H1 and illustrate its utility in the purification of all somatic variants and one germline variant.

Tue Jan 22 00:00:00 EST 2019

Bioinformatics

Protein Aliases: H1 antibody; H1 histone family, member 0; H1 histone family, member 1; H1 histone family, member 2; H1 histone family, member 5; H1 VAR.1; H1 VAR.3; H1 VAR.5; H1(0); H1-0; H1-0 histone; H1.0; H1.0, H1(0), H1-0; H10; H1a; H1b; H1c; H1F3; H1FV; HIST1H1B antibody; HIST1H1D; Histone 1 antibody; histone 1, family 5; histone 1, H1a; histone 1, H1b; histone 1, H1c; histone H1; Histone H1'; Histone H1(0); Histone H1-0; Histone H1.0; Histone H1.0, N-terminally processed; Histone H1.1; Histone H1.2; Histone H1.5; Histone H1a; Histone H1b; Histone H1c; Histone H1d; Histone H1s-1; Histone H1s-3; MyD196

Gene Aliases: 0610008C09Rik; D130017D06Rik; H1; H1(0); H1-0; H1-1; H1-2; H1-5; H1.1; H1.2; H1.5; H10; H1A; H1B; H1C; H1F0; H1F1; H1F2; H1F5; H1FV; H1s-1; H1s-3; H1var1; H1var3; HIf5; His1a; HIST1; HIST1H1A; HIST1H1B; HIST1H1C

UniProt ID: (Human) P07305, (Mouse) P10922, (Rat) P43278, (Human) Q02539, (Human) P16401, (Human) P16403, (Mouse) P43275, (Rat) D4A3K5, (Mouse) P43276, (Rat) D3ZBN0, (Mouse) P15864