Invitrogen

Histone H1 Polyclonal Antibody

Product Image Gallery

Histone H1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Histone H1 was performed using 70% confluent log phase U-2OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone H1 Rabbit Polyclonal Antibody (Product # PA5-30055) at 5 microgram/mL concentration in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Histone H1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Histone H1 Polyclonal Antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H1 Polyclonal Antibody (Product # PA5-30055) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Histone H1 Antibody in Western Blot (WB) — Western blot analysis was performed on acid extracts (20 µg lysate) of NTERA-2 (Lane 1), U-2 OS (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5) and LNCaP (Lane 6). The blot was probed with Anti-Histone H1 (Product # PA5-30055, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 30 kDa band corresponding to Histone H1 was observed across the cell lines tested.

Product Details

PA5-30055

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:20,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 1 and 194 of Human Histone H1
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547529

Product Specific Information

Recommended positive controls: HepG2, HepG2 nuclear extract, mouse liver.

Predicted reactivity: Mouse (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This locus is the ortholog of a mouse protein-coding gene that displays characteristics of a linker histone and is expressed in nuclei of late maturing spermatids. The human locus is expressed; however, the open reading frame has been disrupted by a frameshift so it is unlikely to encode a functional protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The 20S proteasome activator PA28γ controls the compaction of chromatin.

Journal of cell science

Fesquet D,Llères D,Grimaud C,Viganò C,Méchali F,Boulon S,Coux O,Bonne-Andrea C,Baldin V

PA5-30055 was used in Western Blot to show that PA28γ controls chromatin compaction.

Mon Feb 01 00:00:00 EST 2021

Linking epigenetic dysregulation, mitochondrial impairment, and metabolic dysfunction in SBMA motor neurons.

JCI insight

Pourshafie N,Masati E,Bunker E,Nickolls AR,Thepmankorn P,Johnson K,Feng X,Ekins T,Grunseich C,Fischbeck KH

PA5-30055 was used in Western Blotting to show a molecular link between altered epigenetic regulation and mitochondrial metabolism that contributes to neurodegeneration.

Thu Jul 09 00:00:00 EDT 2020

Cx43 and AKAP95 regulate G1/S conversion by competitively binding to cyclin E1/E2 in lung cancer cells.

Thoracic cancer

Chen R,Chen Y,Yuan Y,Zou X,Sun Q,Lin H,Chen X,Liu M,Deng Z,Yao Y,Guo D,Zhang Y

PA5-30055 was used in western blotting to determine how the competitive binding of Cx43 and AKAP95 to cyclin E1/E2 affects G1/S conversion in lung cancer cells.

Mon Jun 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: H1 antibody; H1 histone family, member 1; H1 histone family, member 2; H1 histone family, member 5; H1 VAR.1; H1 VAR.3; H1 VAR.5; H1(0); H1-0; H1.0; H10; H1a; H1b; H1c; H1F3; H1FV; HIST1H1B antibody; HIST1H1D; Histone 1 antibody; histone 1, family 5; histone 1, H1a; histone 1, H1b; histone 1, H1c; histone H1; histone H1'; Histone H1.1; Histone H1.2; Histone H1.5; Histone H1a; Histone H1b; Histone H1c; Histone H1d; Histone H1s-1; Histone H1s-3

Gene Aliases: 0610008C09Rik; H1; H1-1; H1-2; H1-5; H1.1; H1.2; H1.5; H1A; H1B; H1C; H1F1; H1F2; H1F5; H1s-1; H1s-3; H1var1; H1var3; HIf5; His1a; HIST1; HIST1H1A; HIST1H1B; HIST1H1C

UniProt ID: (Human) Q02539, (Human) P16401, (Human) P16403, (Mouse) P43275, (Rat) D4A3K5, (Mouse) P43276, (Rat) D3ZBN0, (Mouse) P15864

Entrez Gene ID: (Human) 3024, (Human) 3009, (Human) 3006, (Mouse) 80838, (Rat) 291145, (Mouse) 56702, (Rat) 680522, (Mouse) 50708

Molecular Function: histone