Western blot analysis of fractionated Cos-7 cells using a Histone H2B polyclonal antibody (Product # PA1-41058). Cos-7 cells were fractionated into nuclear cytosolic/membrane fractions. Approximately ~15ug of each fraction was separated on SDS PAGE. Lane 1, Nuclear fraction. Lane 2, Cytosolic/membrane fraction.
|Tested species reactivity||Dog, Chicken, Fruit fly, Human, Mouse, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 111-125 of human histone H2B [GenBank no. CAI19747.1]|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila.
Suggested positive control: Jurkat, PBMC.
Histones are relatively small proteins that contain a high proportion of positively charged amino acids, e.g., arginine and lysine. This positive charge allows histones to bind tightly to the negatively charged DNA. There are five basic types of histone molecules called H1, H2A, H2B, H3, and H4. Histones are abundantly present in virtually all eukaryotic cells. Humsn histone H2B is a 125 amino acid protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.