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Product Details
34-8694-166
| Applications | Tested Dilution | Publications |
|---|---|---|
Neutralization (Neu) |
Assay-Dependent | - |
Functional Assay (FN) |
Assay-Dependent | - |
Control (Ctrl) |
Assay-Dependent | - |
| Product Specifications | |
|---|---|
Species |
Human |
Expression System |
HEK293 cells |
Amino acid sequence |
Human Wnt-7a, amino acids Leu32-Lys349 (Accession # NP_004616.2) |
Molecular weight |
48 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
>80% by SDS-PAGE and RP-HPLC. |
Endotoxin concentration |
<0.1 ng/µg |
Activity |
ED50 = 40-60 ng/mL; determined by the Wnt-3a induced alkaline phosphatase production in MC3T3-E1 cells. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Storage conditions |
-20°C |
Product Specific Information
Description: Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer.
Human Wnt-7a is encoded as a 349 amino acid precursor that, upon secretion, is cleaved into a 318 amino acid (48 kDa) mature form. At the amino acid level, the mature form of human Wnt-7a is approximately 98% identical to mature mouse Wnt-7a. Depending on the tissue, Wnt-7a can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including neurogenesis, muscle regeneration, and carcinogenesis.
Applications Reported: Recombinant human Wnt-7a is biologically active and can inhibit Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells.
Applications Tested: The ED50 of this protein, as determined by Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells, is 40-60 ng/mL.
Source: HEK293 cells (amino acids Leu32-Lys349; accession # NP_004616.2).
Bioactivity: The ED50 of this protein, as determined by inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells, is 40-60 ng/mL.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >80% as demonstrated by SDS-gel electrophoresis and reverse phase HPLC.
Molecular Weight: 48 kDa (mature form).
Storage and handling: For best recovery, quick spin vial prior to opening. Reconstitute in water to a concentration of less than or equal to 0.2 mg/mL. Do not vortex. Use in sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Target Information
Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-7a; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family member 7A; wingless-type MMTV integration site family, member 7A
Gene Aliases: WNT7A
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