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Invitrogen
Human Wnt-7a (Carrier-Free) Recombinant Protein
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Product Details
34-8694-166
Product Specifications
Expression System
HEK293 cells
Class
Recombinant
Type
Protein
Conjugate
Form
Lyophilized
Amount
15 µg
Storage conditions
-20°C
Shipping conditions
Dry ice
Product Specific Information
Description: Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer.
Human Wnt-7a is encoded as a 349 amino acid precursor that, upon secretion, is cleaved into a 318 amino acid (48 kDa) mature form. At the amino acid level, the mature form of human Wnt-7a is approximately 98% identical to mature mouse Wnt-7a. Depending on the tissue, Wnt-7a can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including neurogenesis, muscle regeneration, and carcinogenesis.
Applications Reported: Recombinant human Wnt-7a is biologically active and can inhibit Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells.
Applications Tested: The ED50 of this protein, as determined by Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells, is 40-60 ng/mL.
Source: HEK293 cells (amino acids Leu32-Lys349; accession # NP_004616.2).
Bioactivity: The ED50 of this protein, as determined by inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 cells, is 40-60 ng/mL.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >80% as demonstrated by SDS-gel electrophoresis and reverse phase HPLC.
Molecular Weight: 48 kDa (mature form).
Storage and handling: For best recovery, quick spin vial prior to opening. Reconstitute in water to a concentration of less than or equal to 0.2 mg/mL. Do not vortex. Use in sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Target Information
Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. It is expressed in placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family, ultimately leading to the disruption of β-catenin degradation. Intracellular accumulation of β-catenin increases translocation of the protein into the nucleus, where it binds to TCF/LEF transcription factors to induce the expression of numerous genes. Increased Wnt/β-catenin signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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