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Agrisera
HydA2/Iron-hydrogenase HydA2 Polyclonal Antibody
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FIGURE: 1 / 1
HydA2/Iron-hydrogenase HydA2 Antibody (AS09-600) in WB
50 ng of purified protein (Hyd A 1 and Hyd A 2) were separated on 10% SDS-PAGE and blotted 25 min to PVDF membrane. Filters were blocked 1 h with 3% low-fat milk powder in PBS-T (0.1% TWEEN 20) and probed with anti-Hyd A 2 (AS09 600,1:5,000 over night at 4°C) and secondary anti-rabbit (1:10,000 1 h) antibody (HRP conjugated, manufacture Pierce) in PBS-T containing 3% low fat milk powder. Antibody incubation... View More
Product Details
AS09-600
Product Specifications
Species Reactivity
Algae,
Bacteria
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant, full-length Chlamydomonas reinhardtii HydA-2.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Chlamydomonas reinhardtii, recombinant HydA2 expressed in E.coli
Target Information
HydA2 encodes an oxygen-sensitive, chloroplast-localized [FeFe]-hydrogenase that is induced under anaerobic conditions in the green alga Chlamydomonas reinhardtii. The gene product is synthesized as a nuclear-encoded precursor that is imported into the chloroplast, where it functions in cellular hydrogen metabolism. HydA2 is a ~505 amino-acid enzyme (detected as an ~49 kDa protein in anaerobically induced cells) whose catalytic core contains the conserved motifs of [FeFe]-hydrogenases and houses the H-cluster active site, comprising a [4Fe-4S] subcluster linked to a catalytically active 2Fe subcluster (with CO and CN ligands) that supports reversible proton reduction to molecular hydrogen (H2) and H2 oxidation. Comparative modeling indicates that HydA2 forms a monomeric enzyme with an H2-gas channel architecture similar to bacterial [FeFe]-hydrogenases, while differing by lacking the N-terminal accessory Fe-S centers found in some bacterial enzymes and by containing additional algal-specific sequence insertions. Functionally, genetic analyses show HydA2 can independently catalyze H2 production, contributing-alongside HydA1-to fermentative and photosynthetically driven H2 evolution during anoxia, thereby linking chloroplast redox metabolism to hydrogen turnover.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HYDA2
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