In Western blot applications, this antibody detects a band at ~45kDa. A suggested positive control is mouse lung tissue lysate.
PA5-19889 can be used with blocking peptide PEP-0017.
ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DFF-45; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; ICAD; Inhibitor of CAD; RP23-121D17.3, A330085O09Rik, DFF35, Dff45, ICAD, ICAD-L, ICAD-S
Gene Aliases: A330085O09Rik; DFF35; Dff45; Dffa; ICAD; ICAD-L; ICAD-S
UniProt ID: (Mouse) O54786
Entrez Gene ID: (Mouse) 13347